Prenylated isoflavones from Cudrania tricuspidata inhibit NO production in RAW 264.7 macrophages and suppress HL-60 cells proliferation

  • J Asian Nat Prod Res. 2017 May;19(5):510-518. doi: 10.1080/10286020.2016.1232253.
Hoang Le Tuan Anh  1 ,  Do Thanh Tuan  2 ,  Do Thi Trang  1 ,  Bui Huu Tai  1 ,  Nguyen Xuan Nhiem  1 ,  Pham Hai Yen  1 ,  Phan Van Kiem  1 ,  Chau Van Minh  1 ,  Tran Minh Duc  3 ,  Hee Kyoung Kang  4 ,  Youn Chul Kim  5 ,  Young Ho Kim  6
Affiliations
  • 1. a Institute of Marine Biochemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam.
  • 2. b Department of Biology , Thaibinh Medical University , Thaibinh , Vietnam.
  • 3. c Department of Anatomy , Vietnam University of Traditional Medicine , Hanoi , Vietnam.
  • 4. d School of Medicine and Institute of Medical Science, Jeju University , Jeju 690-756 , Korea.
  • 5. e College of Pharmacy, Wonkwang University , Iksan 570-749 , Korea.
  • 6. f College of Pharmacy, Chungnam National University , Daejeon 34134 , Korea.
PMID: 27649772 DOI: 10.1080/10286020.2016.1232253
Abstract

Inhibitory effects of NO production in RAW 264.7 macrophages guided the isolation of nine prenylated Isoflavones, including a new cudraisoflavone L (1) and eight known metabolites furowanin B (2), erysubin A (3), wighteone (4), lupalbigenin (5), laburnetin (6), isolupalbigenin (7), 6,8-diprenylorobol (8), millewanin H (9) from the leaves of Cudrania tricuspidata. At the concentration of 10 μM, compounds 1, 2, and 4 significantly inhibited NO production with the inhibitory values of 72.5 ± 2.4, 66.9 ± 1.8, and 55.4 ± 2.7%, respectively. In addition, all of isolated compounds 1-9 showed promising cytotoxic effects toward HL-60 cells (IC50 4.3 ± 0.7 to 18.0 ± 1.7 μM).

Keywords
Cudrania tricuspidata; NO inhibitor; Prenylated isoflavone; anti-leukemia; cudraisoflavone L.
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