Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC-MS/MS assay

  • Biomed Chromatogr. 2019 Mar;33(3):e4417. doi: 10.1002/bmc.4417.
Kai Niu  1 ,  Huiyu Yan  2 ,  Chunjie Guo  3 ,  Sixi Zhang  2 ,  Wei Zhu  1 ,  Shiyong Teng  4
Affiliations
  • 1. Department of Otorhinolaryngology Head and Neck Surgery, The First Hospital of Jilin University, Changchun, China.
  • 2. Department of Pharmacy, The First Hospital of Jilin University, Changchun, China.
  • 3. Department of Radiology, The First Hospital of Jilin University, Changchun, China.
  • 4. Department of Anesthesiology, The First Hospital of Jilin University, Changchun, China.
PMID: 30367489 DOI: 10.1002/bmc.4417
Abstract

A highly sensitive LC-MS/MS method was developed to measure oroxin B in rat plasma and tissue homogenates. The analyte and IS were isolated from biological matrices by a simple protein precipitation followed by centrifugation. Detection was conducted by electrospray negative-ionization mass spectrometry in selected-reaction monitoring mode. The assay was linear in the concentration range 4.52-904 ng/mL with intra- and inter-day precision of <14.41%. It was successfully applied to the pharmacokinetics and tissue distribution studies of oroxin B after an intravenous dose of 1.0 mg/kg in rats. The results would be useful for further development of oroxin B.

Keywords
LC-MS/MS; oroxin B; pharmacokinetic study; tissue distribution.
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