Rapid Screening and Characterization of Triterpene Saponins from the Root of Phytolacca acinosa Roxb by High-Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

  • J Chromatogr Sci. 2022 Jan 1;60(1):16-25. doi: 10.1093/chromsci/bmab017.
Yun Ling  1 ,  Yue Zhu  1 ,  Qiao Gan  1 ,  Guilan Li  1 ,  Zefan Luo  1 ,  Yali Pan  1 ,  Lian Zhao  1 ,  Zhineng Lei  1 ,  Xin He  1 ,  Ming Zeng  1 ,  Wenbo Liu  1
Affiliations
  • 1. School of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.
PMID: 33621321 DOI: 10.1093/chromsci/bmab017
Abstract

Triterpene saponins (TSs) are important bioactive constituents with structural diversity widely distributed in many Plants. The root of Phytolacca acinosa Roxb (RPa) has been used as a traditional Chinese medicine. However, TSs as the main active ingredients in RPa have not been fully characterized. Here, we profiled TSs from RPa by high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS). We tentatively identified 29 TSs, including 13 that had not been reported previously from this plant. This study indicates that HPLC-ESI-QTOF-MS/MS is an effective and rapid method for the characterization of complicated TSs in herbal extracts.

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