Recent Technologies for Genetic Code Expansion and their Implications on Synthetic Biology Applications

  • J Mol Biol. 2022 Apr 30;434(8):167382. doi: 10.1016/j.jmb.2021.167382.
Hongting Tang  1 ,  Pan Zhang  1 ,  Xiaozhou Luo  2
Affiliations
  • 1. Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
  • 2. Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. Electronic address: [email protected].
PMID: 34863778 DOI: 10.1016/j.jmb.2021.167382
Abstract

Genetic code expansion (GCE) enables the site-specific incorporation of non-canonical Amino acids as novel building blocks for the investigation and manipulation of proteins. The advancement of genetic code expansion has been benefited from the development of synthetic biology, while genetic code expansion also helps to create more synthetic biology tools. In this review, we summarize recent advances in genetic code expansion brought by synthetic biology progresses, including engineering of the translation machinery, genome-wide codon reassignment, and the biosynthesis of non-canonical Amino acids. We highlight the emerging application of this technology in construction of new synthetic biology parts, circuits, chassis, and products.

Keywords
GCE optimization; non-canonical amino acid biosynthesis; synthetic chassis; synthetic circuits; synthetic parts.
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