[Advances in the structure and function of chitosanase]

  • Sheng Wu Gong Cheng Xue Bao. 2023 Mar 25;39(3):912-929. doi: 10.13345/j.cjb.220530.
Jie Xie  1  2  3 ,  Yubin Li  1  2 ,  Jingwei Liu  1  2  3 ,  Yan Gou  1  2 ,  Ganggang Wang  1  2
Affiliations
  • 1. Key Laboratory of Environmental and Applied Microbiology of Chinese Academy of Sciences, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
  • 2. Key Laboratory of Environmental Microbiology of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
  • 3. University of Chinese Academy of Sciences, Beijing 100049, China.
PMID: 36994562 DOI: 10.13345/j.cjb.220530
Abstract

Chitosanases represent a class of glycoside hydrolases with high catalytic activity on chitosan but nearly no activity on chitin. Chitosanases can convert high molecular weight chitosan into functional chitooligosaccharides with low molecular weight. In recent years, remarkable progress has been made in the research on chitosanases. This review summarizes and discusses its biochemical properties, crystal structures, catalytic mechanisms, and protein engineering, highlighting the preparation of pure chitooligosaccharides by enzymatic hydrolysis. This review may advance the understandings on the mechanism of chitosanases and promote its industrial applications.

Keywords
catalytic mechanism; chitosanase; crystal structure; rational design.
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