Animal-Free IL-12 alpha Protein, Mouse (His)

IL-12 alpha protein forms two cytokines: IL-12 and IL-35. It modulates T cell and natural killer cell responses, induces IFN-γ production, and promotes Th1 cell differentiation. Animal-Free IL-12 alpha Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-12 alpha protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-12 alpha Protein, Mouse (His) is 193 a.a., with molecular weight of ~22.65 kDa.

The IL-12 alpha Protein is capable of heterodimerizing with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is predominantly produced by professional antigen-presenting cells such as B-cells, dendritic cells, macrophages, and granulocytes. It plays a crucial role in regulating T-cell and natural killer-cell responses, inducing interferon-gamma (IFN-gamma) production, promoting the differentiation of T-helper 1 (Th1) cells, and bridging innate resistance with adaptive immunity. The IL-12 receptor, composed of IL12R1 and IL12R2 subunits, mediates the biological effects of IL-12 by initiating the tyrosine phosphorylation of cellular substrates, including TYK2 and JAK2 kinases. The recruited phosphorylated STAT4 translocates to the nucleus, where it regulates the expression of cytokine and growth factor responsive genes. As part of IL-35, IL-12 alpha Protein plays vital roles in maintaining immune homeostasis in the liver microenvironment and serves as an immune-suppressive cytokine. Signaling occurs through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers, requiring the involvement of transcription factors STAT1 and STAT4. The formation of a unique heterodimer between STAT1 and STAT4 enables binding to specific DNA sites. The IL-12 alpha Protein can form disulfide-linked heterodimers with IL12B, known as interleukin IL-12, and non-disulfide-linked heterodimers with EBI3/IL27B, known as interleukin IL-35. Additionally, it interacts with NBR1, promoting IL-12 secretion.

Measure by its ability to induce proliferation in T-cell enriched PBMC. The ED₅₀ for this effect is <0.2 ng/mL.

Mouse

E. coli

C-His

P43431 (R23-A215)

16159  [NCBI]

MRVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA

Approximately 22.65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.