Animal-Free IL-31 Protein, Human (His)
Based on 1 Customer Validation
IL-31 is a multifunctional cytokine that activates STAT3 through IL-31 heterodimeric receptors (IL31RA and OSMR) and may activate STAT1 and STAT5. IL-31 is known for its involvement in skin immunity, where it regulates immune responses. Animal-Free IL-31 Protein, Human (His) is the recombinant human-derived animal-FreeIL-31 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
IL-31 is a multifunctional cytokine that activates STAT3 through IL-31 heterodimeric receptors (IL31RA and OSMR) and may activate STAT1 and STAT5. IL-31 is known for its involvement in skin immunity, where it regulates immune responses. Animal-Free IL-31 Protein, Human (His) is the recombinant human-derived animal-FreeIL-31 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
Background
IL-31, a cytokine with diverse functions, acts by activating STAT3, and potentially STAT1 and STAT5, utilizing the IL-31 heterodimeric receptor formed by IL31RA and OSMR. This activation mechanism has been implicated in skin immunity, highlighting its role in modulating immune responses. Additionally, IL-31 exhibits the capacity to enhance myeloid progenitor cell survival in vitro, emphasizing its involvement in cellular processes beyond immune regulation. Furthermore, IL-31 induces the expression of key genes, such as RETNLA and serum amyloid A protein, in macrophages, suggesting a broader impact on inflammatory and immune-related pathways.
Verified Bioactivity
Measured by its ability to induce STAT3 activation in U-87 cells. The ED50 for this effect is <20 ng/mL.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
Q6EBC2 (S24-T164)
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Molecular Construction
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N-term
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IL-31 (S24-T164)
Accession # Q6EBC2 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IL31; IL-31; Interleukin 31; Interleukin-31
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AA Sequence
SHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT
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Predicted Molecular Mass
16.8 kDa
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Molecular Weight
Approximately 18 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, trehalose.
<0.1 EU per 1 μg of the protein by the LAL method.
It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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