IL-17A-17F Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi)

IL-17A-17F Heterodimer Protein is the heterodimer of the cytokines IL-17A and IL-17F. Both IL-17A and IL-17F can induce antimicrobial peptides, cytokines (IL-6 and GM-CSF), chemokines (CCL2, CCL7 and CXCL1), and matrix metalloproteinases (MMP-1 and MMP13). IL-17A-17F shows intermediate biological activity between IL-17A and IL-17F^[1][2][3][4]. IL-17A-17F Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant human IL-17A-17F heterodimer protein with His tag and Avi tag at the C-terminus and is expressed in HEK293 cells. It consists of 265 amino acids (IL-17A (G24-A155) & IL-17F (R31-Q163)).

IL-17A-17F Heterodimer Protein is the heterodimer of the cytokines IL-17A and IL-17F. Both IL-17A and IL-17F belongs to the IL-17 cytokine family. IL-17A-17F heterodimer, IL-17A and IL-17F homodimers can be produced by differentiated Th17 cells^[1][2]. IL-17F shares the most similarities with IL-17A (50% homology)^[2]. Both IL-17A and IL-17F can induces antimicrobial peptides, cytokines (IL-6 and GM-CSF), chemokines (CCL2, CCL7 and CXCL1), and matrix metalloproteinases (MMP-1 and MMP13)^[2][3]. IL-17A, IL-17F and IL-17A-17F use the same receptor complex: IL-17RA and IL-17RC heterodimer. And they trigger qualitatively similar signaling pathways. IL-17A-17F shows intermediate biological activity between IL-17A (most potent) and IL-17F (least potent)^[2][4].

IL-17F/IL-17A (5 ng/mL; 16-24 h) induces GRO-, IL-6, and IL-8 secretion in human primary foreskin fibroblast (BJ) cells^[5].
IL-17F/IL-17A binds to IL-17RA.Fc with an EC₅ of 329 ng/mL, IL-17A and IL-17F bind to IL-17RA.Fc with EC₅s of 19 ng/mL and > 2 ng/mL, respectively^[5].

Human

HEK293

C-Avi;C-His

Q16552 (G24-A155)&Q96PD4 (R31-Q163)

3605  [NCBI]&112744  [NCBI]

Approximately 16 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Chen K, et al. Interluekin-17A (IL17A). Gene. 2017 May 30;614:8-14.  [Content Brief]

  [2]. Chang SH, et al. IL-17F: regulation, signaling and function in inflammation. Cytokine. 2009 Apr;46(1):7-11.  [Content Brief]

  [3]. Iwakura Y, et al. The roles of IL-17A in inflammatory immune responses and host defense against pathogens. Immunol Rev. 2008 Dec;226:57-79.  [Content Brief]

  [4]. McGeachy MJ, et al. The IL-17 Family of Cytokines in Health and Disease. Immunity. 2019 Apr 16;50(4):892-906.  [Content Brief]

  [5]. Wright JF, et al. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J Immunol. 2008 Aug 15;181(4):2799-805.  [Content Brief]