IL-2 Protein, Human (C145S)

IL-2 protein, primarily produced by activated CD4-positive helper T-cells, plays vital roles in immune response and tolerance. Binding to IL-2R induces downstream signaling through phosphorylation of JAK1 and JAK3, leading to activation of various pathways, including STAT, PI3K, and MAPK. IL-2 serves as a T-cell growth factor, enhances NK-cell cytolytic activity, and promotes B-cell proliferation. It crucially regulates regulatory T-cells, maintaining immune tolerance, and participates in the differentiation and homeostasis of various T-cell subsets. IL-2 Protein, Human (C145S) is the recombinant human-derived IL-2 protein, expressed by E. coli , with tag free. and C145S mutation. The total length of IL-2 Protein, Human (C145S) is 132 a.a., with molecular weight of ~14.0 kDa.

The cytokine interleukin-2 (IL-2), produced primarily by activated CD4-positive helper T-cells and, to a lesser extent, by activated CD8-positive T-cells and natural killer (NK) cells, plays pivotal roles in the immune response and tolerance. IL-2 binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122, and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). This interaction induces oligomerization and conformational changes in the IL-2R subunits, initiating downstream signaling with the phosphorylation of JAK1 and JAK3. Subsequently, JAK1 and JAK3 phosphorylate the receptor, creating a docking site for the phosphorylation of various substrates, including STAT5. This process activates multiple pathways, including STAT, phosphoinositide-3-kinase/PI3K, and mitogen-activated protein kinase/MAPK pathways. IL-2 functions as a T-cell growth factor, enhances NK-cell cytolytic activity, and promotes robust proliferation of activated B-cells, leading to increased immunoglobulin production. Furthermore, IL-2 plays a crucial role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, essential for maintaining immune tolerance. Additionally, IL-2 participates in the differentiation and homeostasis of various effector T-cell subsets, including Th1, Th2, Th17, as well as memory CD8-positive T-cells.

The cell proliferation assay using CTLL-2 mouse cytotoxic T cells has an ED₅₀ value of 90-270 pg/mL.

Human

E. coli

Tag Free

P60568 (P22-T153,C145S)

3558  [NCBI]

PTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFSQSIISTLT

Approximately 14.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 10 mM Acetata-Na, 5% Trehalose, pH 4.5 .

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.