1. Recombinant Proteins
  2. IL-4 Protein, Human (HEK293)

IL-4 Protein, Human (HEK293)

Cat. No.: HY-P70750
Handling Instructions

IL-4 Protein, Human (HEK293) is a potent lymphoid cell growth factor, which stimulates the growth and survivability of B-cells and T-cells. IL-4 Protein, Human (HEK293) is a recombinant human interleukin-4 (rhIL-4) expressed in HEK 293 cells.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $230 In-stock
Estimated Time of Arrival: December 31
50 μg $690 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
Species

Human

Source

HEK 293

Accession

P05112 (H25-S153)

Gene ID

3565

Molecular Weight

Approximately 18.0 kDa

AA Sequence

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity
The cell proliferation assay using TF-1 human erythroleukemic cells has an ED50 value of 0.01-0.05 ng/mL. Cynomolgus IL-4RA-Fc can bind Human IL-4 with an affinity constant of 0.54 nM.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Background

Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13. IL-4, originally designated as B-cell growth factor-1 (BSFl), is described in the supernatant of activated EL-4 thymoma cells as a factor that can co-stimulate B-cells activated with submitogenic concentrations of anti-IgM[1].

Documentation
References
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Product Name:
IL-4 Protein, Human (HEK293)
Cat. No.:
HY-P70750
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