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IL-4 Protein, Human (HEK293)

4 Cited Publications
Customer Review

Based on 4 publication(s) in Google Scholar

IL-4 Protein, Human (HEK293) is a potent lymphoid cell growth factor, which stimulates the growth and survivability of B-cells and T-cells. IL-4 Protein, Human (HEK293) is a recombinant human interleukin-4 (rhIL-4) expressed in HEK293 cells.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-4 Protein, Human (HEK293)
  • Experimental Validation Results for IL-4 Protein, Human (HEK293)
  • Experimental Validation Results for IL-4 Protein, Human (HEK293)
  • Experimental Validation Results for IL-4 Protein, Human (HEK293)
  • IL-4 Protein, Human (HEK293) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Top Publications Citing

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Biological Activity

Description

IL-4 Protein, Human (HEK293) is a potent lymphoid cell growth factor, which stimulates the growth and survivability of B-cells and T-cells. IL-4 Protein, Human (HEK293) is a recombinant human interleukin-4 (rhIL-4) expressed in HEK293 cells.

Background

Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13. IL-4, originally designated as B-cell growth factor-1 (BSFl), is described in the supernatant of activated EL-4 thymoma cells as a factor that can co-stimulate B-cells activated with submitogenic concentrations of anti-IgM[1].

Verified Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ≤0.247 ng/mL, corresponding to a specific activity is ≥4.049×106 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag Tag Free
  • Accession

    P05112-1 (H25-S153)

  • Gene ID
    3565  [NCBI]
  • Molecular Construction
    • N-term
    • IL-4 (H25-S153)
      Accession # P05112-1
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    IL4; B_cell Stimulatory Factor 1; Interleukin 4; B-Cell Stimulatory Factor 1; IL-4; B Cell Growth Factor 1; Lymphocyte Stimulatory Factor 1; Binetrakin; Interleukin-4; Pitrakinra; BCGF-1; MGC79402; BCGF1; BSF-1; BSF1

  • AA Sequence

    HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

  • Predicted Molecular Mass

    15 kDa

  • Molecular Weight

    Approximately 19-25 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Le HV, et al. Isolation and characterization of multiple variants of recombinant human interleukin 4 expressed in mammalian cells. J Biol Chem. 1988 Aug 5;263(22):10817-23. [Content Brief]

[2]. Melih Ö Celik, et al. IL-4 induces M2 macrophages to produce sustained analgesia via opioids. JCI Insight. 2020 Feb 27;5(4):e133093. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1732 ng/mL, corresponding to a specific activity is 5.774×106 U/mg

  • 1/16

    IL-4 Protein, Human (HEK293) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 May 16:155:114632. [Abstract]

    Customer Validation
     After induction with IL-4 and IL-13, differential expression of KLF15 mRNA in macrophages of PMA, M2, M2-NC, and M2-KLF15 groups.

  • 1/16

    IL-4 Protein, Human (HEK293) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 May 16:155:114632. [Abstract]

    Customer Validation
     After induction with IL-4 and IL-13, differential expression of KLF15 protein in macrophages of PMA, M2, M2-NC, and M2-KLF15 groups.

  • IL-4 Protein, Human (HEK293) Product Image
    1/16
  • Experimental Validation Results for IL-4 Protein, Human (HEK293)
  • Experimental Validation Results for IL-4 Protein, Human (HEK293)
  • Experimental Validation Results for IL-4 Protein, Human (HEK293)
  • Experimental Validation Results for IL-4 Protein, Human (HEK293)
  • IL-4 Protein, Human (HEK293) Product Image