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Wnt3a Surrogate Protein, Human (HEK293, Fc)

Cat. No.: HY-P70453B
COA Handling Instructions

Wnt3a Surrogate protein is a Wnt recombination protein that plays an important role in embryonic development, adult tissue homeostasis and injury repair. Wnt3a Surrogate protein, consisting of the domain and Fc portion of Wnt3a, helps organoids expand and maintain a source of Wnt. Organoids are a rapidly evolving model system for tissue development, and Wnt3a is considered as the essential protein for organoid growth. Wnt3a or the small molecule glycogen synthase kinase 3 (GSK3) inhibitor CHIR are commonly used as Wnt sources in experiments. However, the application of Wnt3a is hampered by Wnt lipidation, and cross-reactivity with Wnt frizzled (Fzd). Therefore, Wnt3a Surrogate protein was used instead of Wnt3a, as a water-soluble and Fzd-specific alternative Wnt agonist. And the Wnt3a Surrogate protein exhibits tissue selectivity consistent with Fzd isoform specificity. Wnt3a Surrogate Protein, Human (HEK293, Fc) is a recombinant Wnt3a protein of human origin. It is expressed by HEK293 cells with an Fc tag.

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Description

Wnt3a Surrogate protein is a Wnt recombination protein that plays an important role in embryonic development, adult tissue homeostasis and injury repair. Wnt3a Surrogate protein, consisting of the domain and Fc portion of Wnt3a, helps organoids expand and maintain a source of Wnt. Organoids are a rapidly evolving model system for tissue development, and Wnt3a is considered as the essential protein for organoid growth. Wnt3a or the small molecule glycogen synthase kinase 3 (GSK3) inhibitor CHIR are commonly used as Wnt sources in experiments. However, the application of Wnt3a is hampered by Wnt lipidation, and cross-reactivity with Wnt frizzled (Fzd). Therefore, Wnt3a Surrogate protein was used instead of Wnt3a, as a water-soluble and Fzd-specific alternative Wnt agonist. And the Wnt3a Surrogate protein exhibits tissue selectivity consistent with Fzd isoform specificity[1][2][3]. Wnt3a Surrogate Protein, Human (HEK293, Fc) is a recombinant Wnt3a protein of human origin. It is expressed by HEK293 cells with an Fc tag.

Background

Wnt is a secreted glycoprotein comprising 19 human proteins that signal through both canonical and non-canonical pathways. Wnts are involved in developmental morphology, stem cell maintenance, and wound healing, and can travel long distances or attach to the surface of exosomes. Among them, Wnt3a is an important molecule that can activate the Wnt/β-catenin signaling pathway and participate in various physiological and pathological processes, such as embryonic development, tissue repair, and tumor progression. Wnt3a also plays a role in cardiomyogenesis, skeletal muscle development, nervous system formation, and migration and invasion of trophoblast cells[4][5].
1. The Wnt family includes: Wnt1, Wnt2, Wnt2b (Wnt13), Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt5b, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a (Wnt14), Wnt9b (Wnt14b), Wnt10a, Wnt10b, Wnt11 and Wnt16.
2. Wnt canonical pathway: also known as Wnt/β-catenin pathway.
3. Wnt non-canonical pathway: including Wnt-planar cell polarity pathway (Wnt-PCP pathway) and Wnt-calcium pathway (Wnt-Ca2+ pathway).
4. Introduction to Wnt3a: It is an important molecule in the process of cell signal transduction and development, and is highly conserved. Wnt3a interacts with Frizzled receptors, induces the accumulation of β-catenin, and activates the Wnt/β-catenin signaling pathway. Wnt3a also stimulates migration and invasion of trophoblasts.
Wnt3a function: In embryonic development, Wnt3a is involved in the formation of embryonic axis and directed cell differentiation; in adult tissues, Wnt3a is related to the regulation of stem cell self-renewal and cell fate determination, and is also related to the occurrence and development of certain tumors. For example, Wnt3a promotes or inhibits tumor progression through the canonical Wnt signaling pathway. Wnt3a is also associated with post-injury remodeling and cell expansion, and upregulation of WNT3a improves repair. Wnt3a also plays an important role in organ formation. It can promote cardiomyogenesis by increasing T cell-specific transcription factor (TCF)-dependent transcription and inducing cardiac-specific markers, bone morphogenetic proteins (BMPs) and myosin heavy chain (MHC). Studies have shown that Wnt3a induces myogenesis in differentiated body muscle groups, activates myogenic differentiation antigen (MyoD) expression, and promotes skeletal muscle development. Wnt3a activates the atypical protein kinase Cloys (pKCiota) and induces a punctate distribution of pKCiota in neurites and cytoplasm, with a particularly strong signal at the centrosome. Wnt3a plays a crucial role in the maintenance and differentiation of neuromesoderm stem cells. Thus, Wnt3a is involved in the formation of the nervous system.

Biological Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <62 ng/mL.

Species

Human

Source

HEK293

Tag

Fc

Accession

/

Gene ID

/

Synonyms
Wnt3a Surrogate, Human (HEK293, Fc)
Molecular Weight

66-72 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 500 mM NaCl, pH 8.2.

Endotoxin Level

< 0.01 EU/µg as determined by LAL test.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

Wnt3a Surrogate Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Wnt3a Surrogate Protein, Human (HEK293, Fc)
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HY-P70453B
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