Rhodizonic acid disodium  (Synonyms: Sodium rhodizonate dibasic)

Rhodizonic acid disodium (Sodium rhodizonate dibasic) is a transition metal-dependent pro-oxidant and lead detection agent that induces reactive oxygen species generation, DNA damage, and inhibits Aconitase activity. Rhodizonic acid disodium generates superoxide anion radicals in an iron (II)-dependent manner, leading to aconitase inactivation. Rhodizonic acid disodium also triggers hydroxyl radical-mediated DNA strand breaks and 8-OHdG formation via copper ion reduction. Rhodizonic acid disodium reacts with lead to form a scarlet precipitate, with the color intensity proportional to lead content, enabling qualitative or quantitative analysis of lead. Rhodizonic acid disodium can also be used for real-time visualization of the dynamic process of lead sequestration in the plant rhizosphere and evaluation of the effects of environmental factors such as soil type on the stability of lead-sequestering structures^[1][2].

Disodium rhodizonate (5 mg) serves as a highly efficient real-time staining agent for detecting lead sequestration by living root border cells and their mucilage in Golden Bantam maize. It forms a scarlet precipitate proportional to lead content, while showing pale yellow staining under lead-free conditions^[2].
Procedure: Staining for 10 sec; lead exposure for 1 min/60 min; incubation with lead + nuclease for 60 min; nuclease incubation for 20 min after lead treatment^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

214.04

C₆Na₂O₆

523-21-7

Solid

Brown to black

O=C(C(O[Na])=C(C1=O)O[Na])C(C1=O)=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Murakami K, et al. Prooxidant action of rhodizonic acid: transition metal-dependent generation of reactive oxygen species causing the formation of 8-hydroxy-2'-deoxyguanosine formation in DNA. Toxicol In Vitro. 2006;20(6):910-914.

  [2]. Huskey DA, et al. Use of rhodizonic acid for rapid detection of root border cell trapping of lead and reversal of trapping with DNase. Appl Plant Sci. 2019;7(4):e01240. Published 2019 Apr 12.