1. GPCR/G Protein Neuronal Signaling
  2. 5-HT Receptor
  3. Ro60-0175 fumarate

Ro60-0175 fumarate is a potent and selective agonist of 5-HT2C receptor.

For research use only. We do not sell to patients.

Ro60-0175 fumarate Chemical Structure

Ro60-0175 fumarate Chemical Structure

CAS No. : 169675-09-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 254 In-stock
Solution
10 mM * 1 mL in DMSO USD 254 In-stock
Solid
5 mg USD 231 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Ro60-0175 fumarate is a potent and selective agonist of 5-HT2C receptor[1].

IC50 & Target[1]

5-HT2C Receptor

 

In Vivo

Ro60-0175 (1 mg/kg; s.c.) fumarate preserves the regularity of responding seen in control animals in drug-treated group, but drug-treated animals reach their break-points earlier[1].
Ro60-0175 (0.3, 1, and? 3 mg/kg (Ro60-0175); 1?mg/kg (yohimbine); s.c.; i.p.) fumarate dose dependently attenuates the response increased by the yohimbine treatment alone relative to vehicle injection[1].
Ro60-0175 (0.5 mg/kg (SB242084); 1?mg/kg (Ro60-0175); 1?mg/kg (yohimbine); s.c.; i.p.) fumarate reduces responding and that this effect is prevented by SB242084 pretreatment[1].
Ro60-0175 (0.3, 1, and?3 mg/kg; s.c.) fumarate significantly reduces responding on the active lever in the reinstatement group[1].
Ro60-0175 (0.5?mg/kg SB242084; 1?mg/kg Ro60-0175; s.c.; i.p.) fumarate reduces responding compared to vehicle in the reinstatement group, and that this effect is prevented by pretreatment with SB242084. For responding on the inactive lever, there are no significant main effects or interactions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

342.75

Formula

C15H16ClFN2O4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](C)CN1C=CC2=C1C=C(Cl)C(F)=C2.OC(/C=C/C(O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (291.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9176 mL 14.5879 mL 29.1758 mL
5 mM 0.5835 mL 2.9176 mL 5.8352 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9176 mL 14.5879 mL 29.1758 mL 72.9395 mL
5 mM 0.5835 mL 2.9176 mL 5.8352 mL 14.5879 mL
10 mM 0.2918 mL 1.4588 mL 2.9176 mL 7.2939 mL
15 mM 0.1945 mL 0.9725 mL 1.9451 mL 4.8626 mL
20 mM 0.1459 mL 0.7294 mL 1.4588 mL 3.6470 mL
25 mM 0.1167 mL 0.5835 mL 1.1670 mL 2.9176 mL
30 mM 0.0973 mL 0.4863 mL 0.9725 mL 2.4313 mL
40 mM 0.0729 mL 0.3647 mL 0.7294 mL 1.8235 mL
50 mM 0.0584 mL 0.2918 mL 0.5835 mL 1.4588 mL
60 mM 0.0486 mL 0.2431 mL 0.4863 mL 1.2157 mL
80 mM 0.0365 mL 0.1823 mL 0.3647 mL 0.9117 mL
100 mM 0.0292 mL 0.1459 mL 0.2918 mL 0.7294 mL
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Ro60-0175 fumarate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Ro60-0175 fumarate
Cat. No.:
HY-103140
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