Art. -Nr.: HY-103140

Ro60-0175 fumarate

Name: Ro60-0175 fumarate
Brand: MedChemExpress
SKU: HY-103140
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Ro60-0175 fumarate is a potent and selective agonist of 5-HT_2C receptor.

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Reinheit : 99.47%
CAS. Nr.: 169675-09-6
Formel: C₁₅H₁₆ClFN₂O₄
Molecular Weight:342.75

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Ro60-0175 fumarate Chemical Structure

CAS. Nr.: 169675-09-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Ro60-0175 fumarate is a potent and selective agonist of 5-HT_2C receptor^[1].

IC₅₀ & Target

^[1]

5-HT_2C Receptor

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	0.0047 μM Compound: Ro 60-0175	Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay Agonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay	[PMID: 23301527]
HEK293	EC₅₀	0.027 μM Compound: Ro 60-0175	Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay Agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as induction of intracellular calcium release measured for 90 secs by fluorescence assay	[PMID: 23301527]

In Vivo

Ro60-0175 (1 mg/kg; s.c.) fumarate preserves the regularity of responding seen in control animals in drug-treated group, but drug-treated animals reach their break-points earlier^[1].
Ro60-0175 (0.3, 1, and 3 mg/kg (Ro60-0175); 1 mg/kg (yohimbine); s.c.; i.p.) fumarate dose dependently attenuates the response increased by the yohimbine treatment alone relative to vehicle injection^[1].
Ro60-0175 (0.5 mg/kg (SB242084); 1 mg/kg (Ro60-0175); 1 mg/kg (yohimbine); s.c.; i.p.) fumarate reduces responding and that this effect is prevented by SB242084 pretreatment^[1].
Ro60-0175 (0.3, 1, and 3 mg/kg; s.c.) fumarate significantly reduces responding on the active lever in the reinstatement group^[1].
Ro60-0175 (0.5 mg/kg SB242084; 1 mg/kg Ro60-0175; s.c.; i.p.) fumarate reduces responding compared to vehicle in the reinstatement group, and that this effect is prevented by pretreatment with SB242084. For responding on the inactive lever, there are no significant main effects or interactions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 169675-09-6
Appearance Solid
Molecular Weight 342.75
Formel C₁₅H₁₆ClFN₂O₄
Color White to off-white
SMILES

N[C@@H](C)CN1C=CC2=C1C=C(Cl)C(F)=C2.OC(/C=C/C(O)=O)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (291.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9176 mL

14.5879 mL

29.1758 mL

5 mM

0.5835 mL

2.9176 mL

5.8352 mL

10 mM

0.2918 mL

1.4588 mL

2.9176 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Fletcher PJ, et al. The 5-HT2C receptor agonist Ro60-0175 reduces cocaine self-administration and reinstatement induced by the stressor yohimbine, and contextual cues. Neuropsychopharmacology. 2008;33(6):1402-1412. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9176 mL	14.5879 mL	29.1758 mL	72.9395 mL
	5 mM	0.5835 mL	2.9176 mL	5.8352 mL	14.5879 mL
	10 mM	0.2918 mL	1.4588 mL	2.9176 mL	7.2939 mL
	15 mM	0.1945 mL	0.9725 mL	1.9451 mL	4.8626 mL
	20 mM	0.1459 mL	0.7294 mL	1.4588 mL	3.6470 mL
	25 mM	0.1167 mL	0.5835 mL	1.1670 mL	2.9176 mL
	30 mM	0.0973 mL	0.4863 mL	0.9725 mL	2.4313 mL
	40 mM	0.0729 mL	0.3647 mL	0.7294 mL	1.8235 mL
	50 mM	0.0584 mL	0.2918 mL	0.5835 mL	1.4588 mL
	60 mM	0.0486 mL	0.2431 mL	0.4863 mL	1.2157 mL
	80 mM	0.0365 mL	0.1823 mL	0.3647 mL	0.9117 mL
	100 mM	0.0292 mL	0.1459 mL	0.2918 mL	0.7294 mL

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Ro60-0175 fumarate

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen