RUNX1/ETO-IN-1
Based on 1 Customer Validation
RUNX1/ETO-IN-1 is a RUNX1-ETO oncogenic fusion protein inhibitor that specifically targets the NHR2 oligomerization domain. RUNX1/ETO-IN-1 directly interacts with the NHR2 (KD,app = 39 μM). RUNX1/ETO-IN-1 exhibits anti-leukemic activity by inducing apoptosis and promoting differentiation in RUNX1/ETO-translocated AML cells. RUNX1/ETO-IN-1 remains essentially uncharged at physiological pH, demonstrating superior membrane permeability.
For research use only. We do not sell to patients.
- Purity: 91.18%
- CAS No.: 923865-06-9
- Formula: C21H25N3O3S
- Molecular Weight:399.51
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
RUNX1/ETO-IN-1 (Cmpound M23) (96 h) Inhibits cell viability with an IC50 of 62.14 µM in the AML cell line SKNO-1 carrying the t(8;21) translocation that results in RUNX1/ETO[1].
RUNX1/ETO-IN-1 (20-200 µM) reduces the thermal stability of NHR2 (ΔTm = -1.6°C) in a concentration-dependent manner, likely by interfering with the tetramer-dimer equilibrium[1].
RUNX1/ETO-IN-1 (50-100 µM, 24 h) arrests cell cycle and potential induction of apoptosis in SKNO-1 cells, but not in THP-1 cells[1].
RUNX1/ETO-IN-1 (50-100 µM, 24 h) induces apoptosis in SKNO-1 cells by increasing caspase-3 and -7 activity but not in THP-1 and healthy NC-NC control cells[1].
RUNX1/ETO-IN-1 (25-50 µM, 3 days) promotes differentiation in SKNO-1 cells by increasing CD11b expression, but not in K562 and THP-1 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RUNX1/ETO-negative THP-1, K562, HL-60 cells, RUNX1/ETO-dependent SKNO-1 cells, B-lymphoblastoid cells
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Concentration:1-100 μM
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Incubation Time:72 h
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Result:Showed the highest sensitivity to SKNO-1 cells
Affected RUNX1/ETO-negative THP-1, K562, and HL-60 cells slightly
Did not affect B-lymphoblastoid cells
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Cell Line:RUNX1/ETO-negative THP-1 and RUNX1/ETO-positive SKNO-1 cells
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Concentration:50, 100 μM
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Incubation Time:24 h
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Result:Induced an accumulation in the subG1 and G1 phases, along with a reduction in the S and G2/M phases in the RUNX1/ETO-positive SKNO-1 cells and did not affect the cell cycle phase in the RUNX1/ETO-negative THP-1 cells.
Chemical Information
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CAS No. 923865-06-9
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Appearance Solid
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Molecular Weight 399.51
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Formula C21H25N3O3S
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Color Light yellow to brown
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SMILES
CC1=CSC2=NC(CNC(C(C)C)C3=CC4=C(OCCCO4)C=C3)=CC(N12)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 25 mg/mL (62.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5031 mL | 12.5153 mL | 25.0307 mL | 62.5767 mL |
| 5 mM | 0.5006 mL | 2.5031 mL | 5.0061 mL | 12.5153 mL | |
| 10 mM | 0.2503 mL | 1.2515 mL | 2.5031 mL | 6.2577 mL | |
| 15 mM | 0.1669 mL | 0.8344 mL | 1.6687 mL | 4.1718 mL | |
| 20 mM | 0.1252 mL | 0.6258 mL | 1.2515 mL | 3.1288 mL | |
| 25 mM | 0.1001 mL | 0.5006 mL | 1.0012 mL | 2.5031 mL | |
| 30 mM | 0.0834 mL | 0.4172 mL | 0.8344 mL | 2.0859 mL | |
| 40 mM | 0.0626 mL | 0.3129 mL | 0.6258 mL | 1.5644 mL | |
| 50 mM | 0.0501 mL | 0.2503 mL | 0.5006 mL | 1.2515 mL | |
| 60 mM | 0.0417 mL | 0.2086 mL | 0.4172 mL | 1.0429 mL |
RUNX1/ETO-IN-1 Related Classifications
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