sBADA TFA 

sBADA TFA is a potent green fluorescent dye. sBADA TFA is a sulfonated BODIPY-FL 3-amino-D-alanine. sBADA TFA is used to label peptidoglycans in bacterial cell walls in situ. (λ_ex=490 nm, λ_em=510 nm)^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
sBADA TFA assay^[1] (to quantify GAPDH):
1. Wash S. venezuelae cells twice with pre‐warms fresh LB broth (30°C) and collects via centrifuge (7000 g, 2 minutes).
2. S. venezuelae cells are re-suspended in fresh LB broth (30°C) containing 1mM sBADA TFA and cultured for 5 minutes.
3. S. venezuelae cells are treated with 70% ice-cold ethanol and incubates on ice-bath for 1 hour.
4. The fixed cells are then washed twice with 4°C 1x PBS via centrifuge and re‐suspends in 4°C 1x PBS before imaging.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

594.25

C₁₉H₂₁BF₅N₄NaO₈S

510

490

CC1=CC(C)=C2C=C3C=C(C(CCC(NC[C@H](C(O)=O)N)=O)=[N+]3[B-](F)(N12)F)S(=O)(O[Na])=O.OC(C(F)(F)F)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Hsu YP, et, al. Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem Sci. 2017 Sep 1;8(9):6313-6321.  [Content Brief]