1. Dye Reagents
  2. sBADA

sBADA 

Cat. No.: HY-D1690
Handling Instructions

sBADA is a potent green fluorescent dye. sBADA is a sulfonated BODIPY-FL 3-amino-D-alanine. sBADA is used to label peptidoglycans in bacterial cell walls in situ. (λex=490 nm, λem=510 nm).

For research use only. We do not sell to patients.

sBADA Chemical Structure

sBADA Chemical Structure

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Description

sBADA is a potent green fluorescent dye. sBADA is a sulfonated BODIPY-FL 3-amino-D-alanine. sBADA is used to label peptidoglycans in bacterial cell walls in situ. (λex=490 nm, λem=510 nm)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
sBADA assay[1] (to quantify GAPDH):
1. Wash S. venezuelae cells twice with pre‐warms fresh LB broth (30 ℃) and collects via centrifuge (7000 g, 2 minutes).
2. S. venezuelae cells are re-suspended in fresh LB broth (30 °C) containing 1mM sBADA and cultured for 5 minutes.
3. S. venezuelae cells are treated with 70% ice-cold ethanol and incubates on ice-bath for 1 hour.
4. The fixed cells are then washed twice with 4 ℃ 1x PBS via centrifuge and re‐suspends in 4 ℃ 1x PBS before imaging.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

594.25

Formula

C19H21BF5N4NaO8S

SMILES

CC1=CC(C)=C2C=C3C=C(C(CCC(NC[[email protected]](C(O)=O)N)=O)=[N+]3[B-](F)(N12)F)S(=O)(O[Na])=O.OC(C(F)(F)F)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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sBADA
Cat. No.:
HY-D1690
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