Purification

Environmental pollution refers to the process where human activities release harmful chemicals or energy (such as industrial waste, pesticides, and plastic particles) into the natural environment at a rate that exceeds the ecosystem's capacity for self-purification, leading to deterioration in the composition or state of the atmosphere, water bodies, and soil. Environmental pollution is not only a direct threat to biodiversity but also ultimately impacts human health through the bioaccumulation effect of the food chain. Therefore, the accurate identification and dynamic monitoring of pollutants constitute the scientific cornerstone for formulating environmental policies, assessing ecological risks, and promoting green chemistry transformation.

MCE features a collection of 872 environmental pollutants, covering categories such as air pollutants, water pollutants, and pesticides, for research in the field of environmental chemistry.

The discovery of hit molecule is a cornerstone of drug development. Among the diverse tools available, DNA-encoded libraries have emerged a revolutionary platform for high-throughput screening. Compared with traditional HTS, DEL features shorter screening processes, lower costs, simpler assays, and larger library capacities.

DEL Construction utilizes split-and-pool synthesis, a combinatorial chemistry approach that involves iterative splitting, reaction, and pooling. This strategy enables rapid, exponential assembly of fragments in minimal steps without the need for individual compound synthesis andassoicicated isolation or purification steps, thus greatly reducing overall costs. The technology enables simultaneous affinity screeningof massive compound collections to target proteins in a single step. By coupling chemical structures with unique DNA barcodes, each compound is tagged with a distinct DNA sequence for convenient tracking and decoding.DELs readily enable the construction and efficient screening of libraries containing millions to billions of compounds. As a result, DEL screening combines the dual advantages of high efficiency and low cost, making DEL a transformative technology in modern drug discovery.

The DEL kit consists of 50 independent libraries with a total scale of 100 billion compounds. It is constructed through stepwise combinatorial chemistry strategies involving 2-, 3-, and 4-round synthesis. By employing diverse scaffolds and flexible linking strategies, it encompasses various ring systems, linear frameworks, and heterocyclic structures. Screening can be achieved solely through affinity, independent of target-specific activity detection methods. This library is suitable for DEL screening against a wide range of targets.

MCE High-Affinity Iodoacetyl Agarose, a 4% highly cross-linked agarose reagent coupled with a derivative of iodoacetic acid, is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification.

MCE Ni-NTA His-Tag Purification Agarose, a 6% highly cross-linked agarose reagent, enables high-yield, high-purity purification of his-tagged proteins.

MCE Ni-IDA Agarose can be used for the detection and purification of His-tag proteins.

MCE Glutathione Magnetic Agarose Beads have high protein-binding capacity and stability, making it ideal for high performance purification of GST-tagged fusion proteins expressed in E. coli, yeast, insect and mammalian expression systems.

MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

MCE CHO Magnetic Beads (200 nm, 10 mg/mL) contain CHO functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages，can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.

MCE Protein A/G Agarose is an affinity chromatography medium for separation and purification of immunoglobulins.

MCE Anti-YFP Affinity Gel can be used for the detection and purification of proteins tagged with GFP and EGFP and IP assays.

MCE Anti-GFP Affinity Gel can be used for the detection and purification of GFP, EGFP, their fusion-expressed proteins and IP assays.

MCE Anti-RFP Affinity Gel can be used for the detection and purification of native RFP, RFP mutants, and IP assays.

MCE Anti-HA Magnetic Agarose Beads can be used for the detection and purification of HA fusion-expressed proteins and IP assays.

MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of GFP, EGFP, and their fusion-expressed proteins and IP assays.

MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of Flag (DYKDDDDK) fusion-expressed proteins and IP assays.

MCE Ni-IDA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

MCE Glutathione Agarose, a 4% highly cross-linked agarose reagent, enables high-yield, high-purity purification of GST-tagged proteins.

MCE Ni-NTA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

MCE Protein A Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

MCE Anti-Flag Affinity Gel is used for immunoprecipitation (IP) or protein purification of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE NHS Magnetic Beads (200 nm, 10 mg/mL) contain N-hydroxysuccinimide (NHS) functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages，can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.

MCE Protein G Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

MCE Anti-c-Myc Affinity Gel can be used for the detection and purification of c-Myc fusion expressed proteins, and immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

MCE Anti-HA Affinity Gel can be employed for the detection and purification of HA fusion expressed proteins, and can also be used in immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

MCE IP/Co-IP Kit (Protein A/G Magnetic Beads) can be used for protein purification, IP, Co-IP of target proteins or their protein complexes.

MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems, and also suitable for Co-immunoprecipitation and purification of Flag-tagged protein. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Affinity Chromatography (AC) Columns are designed for purification of recombinant proteins with different tags, enzymes, antibodies, antigens and nucleic acids.

MCE Exosome Isolation and Purification Kit (From Milk) provides a simple and effective method to isolate and purify intact exosomes from milk.

MCE Exosome Isolation and Purification Plus Kit (From Plasma and Serum) provides a simple and effective method to isolate and purify intact exosomes from plasma and serum

MCE Butyl Agarose 4FF is a hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

MCE Octyl Agarose 6FF is a hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

MCE Phenyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules.

MCE Butyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

MCE Octyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling octyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

MCE Protein A Agarose (Alkali-Tolerant) 4FF can efficiently binds IgG from serum, ascites, culture supernatants, and other antibody-containing samples, enabling high-performance antibody purification.

MCE CM Agarose 6FF is a weak cation exchanger formed by covalently coupling carboxymethyl (CM) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

MCE SP Agarose 6FF is a strong cation exchanger formed by covalently coupling sulfopropyl (SP) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

MCE DEAE Agarose 6FF is a weak cation exchanger formed by covalently coupling diethylaminoethyl (DEAE) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

MCE Q Agarose 6FF is a strong cation exchanger formed by covalently coupling quarternary ammonium (Q) groups to agarose. It is suitable for both laboratory research and large-scale industrial purification processes.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from plasma and serum that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cell culture media that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

MCE Blue Agarose 6FF can be used for the purification of biological macromolecules such as albumin, interferons, nucleotide-dependent enzymes (e.g., kinases, dehydrogenases), α2-macroglobulin, and coagulation factors.

MCE Phenyl Agarose (Low Sub) 6FF is a low-substitution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules with relatively weak hydrophobicity.

MCE Phenyl Agarose (High Sub) 6FF is a high-substitution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules with relatively weak hydrophobicity.

MCE Exosome Isolation and Purification Kit (From Tissue) provides a simple and effective method to isolate and purify intact exosomes from a variety of tissues (such as brain, heart, lung, liver, muscle, lymph nodes, thymus, embryo, tumor, etc.).

MCE MBP Agarose (Dextrin) 6FF is prepared by covalently coupling dextrin to an agarose matrix. It features high binding capacity, excellent specificity, and superior ligand stability. It can achieve one-step purification of MBP fusion protein.

MCE Chelating Agarose (IDA) 6FF allows flexible chelation with metal ions such as Zn²⁺, Ni²⁺, or Cu²⁺, and is suitable for the purification of His-tagged recombinant proteins expressed in bacterial, mammalian, insect, and baculovirus systems.

Anti-MBP Magnetic Beads are suitable for the detection and purification of MBP-tagged fusion proteins, as well as for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) applications. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) protects proteins from degradation by endogenous proteases released during protein extraction and purification.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Heparin Agarose 6FF is suitable for the separation and purification of heparin-binding biomolecules, including antithrombin III, coagulation factors, other plasma proteins, DNA-binding proteins, lipoproteins, protein synthesis factors, nucleic acid-related enzymes, and steroid receptors.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cerebro-spinal fluid, amniotic fluid, milk, saliva, etc, which can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.