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  2. FLAG peptide

FLAG peptide 

Cat. No.: HY-P0223 Purity: 99.23%
Handling Instructions

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

FLAG peptide Chemical Structure

FLAG peptide Chemical Structure

CAS No. : 98849-88-8

Size Price Stock Quantity
1 mg USD 50 In-stock
Estimated Time of Arrival: December 31
5 mg USD 70 In-stock
Estimated Time of Arrival: December 31
10 mg USD 120 In-stock
Estimated Time of Arrival: December 31
25 mg USD 280 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

In Vitro

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure[1]. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions[2].

Molecular Weight

1012.97

Formula

C₄₁H₆₀N₁₀O₂₀

CAS No.

98849-88-8

Sequence

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Sequence Shortening

DYKDDDDK

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Stored under nitrogen
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (98.72 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9872 mL 4.9360 mL 9.8720 mL
5 mM 0.1974 mL 0.9872 mL 1.9744 mL
10 mM 0.0987 mL 0.4936 mL 0.9872 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: 99.23%

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Keywords:

FLAG peptideOthersInhibitorinhibitorinhibit

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Product name:
FLAG peptide
Cat. No.:
HY-P0223
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