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pull-down assay

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6

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Fluorescent Dye

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Cat. No. Product Name Target Research Areas Chemical Structure
  • HY-158099

    Celastrol-Biotin

    Biochemical Assay Reagents Cancer
    Biotin-Cel (Celastrol-Biotin) is a biotin-labeled Celastrol (HY-13067). Celastrol exhibits antitumor, anti-inflammatory, and anti-obesity activities. Biotin-Cel can be used in biotin-affinity pulldown assay to identify the molecular target of Celastrol in hepatocellular carcinoma cells .
    Biotin-Cel
  • HY-101974

    Biochemical Assay Reagents Others
    Biotin-PEG3-Bromide is a short PEG linker featuring a biotin group and a bromide. The bromide is a halogen which is easily displaced by nucleophiles such as alcohols or amines. Alternatively, bromide can be applied in a number of cross-coupling reactions such as in a Suzuki reaction. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins.
    Biotin-PEG3-Bromide
  • HY-160841

    DNA Stain DNA/RNA Synthesis Others
    Biotin-16-dCTP is a biotinylated deoxycytidine triphosphate that serves as an important DNA labeling substrate. Biotin-16-dCTP can be enzymatically incorporated into the 3' end of DNA probes via terminal deoxynucleotidyl transferase, forming a 1-3 nucleotide-long tail to achieve biotinylation of the probes. Biotin-16-dCTP enhances chemiluminescent detection of low-abundance targets such as specific tRNA isoacceptors through Northern blotting. Biotin-16-dCTP can also replace conventional dCTP to be integrated into single-stranded DNA generated by asymmetric polymerase chain reaction, which is applicable for bioconjugation or pull-down assays. Repeated freeze-thaw cycles of Biotin-16-dCTP should be avoided to prevent degradation of its function for probe biotinylation .
    Biotin-16-dCTP
  • HY-W615095

    Biochemical Assay Reagents Others
    Biotin-PEG2-alkyne is a short PEG linker featuring a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azides.
    Biotin-PEG2-alkyne
  • HY-W130607

    Biochemical Assay Reagents Others
    Biotinyl-8-amino-3,6-dioxaoctanoic acid is a biotinylating reagent linked with a PEG chain for improved water solubility. Biotin is an affinity ligand and it is used in biochemical applications such as pull-down assays or for ligating with streptavidin proteins. The carboxylic group can react with amine-containing molecules in the presence of activators such as HATU.
    Biotinyl-8-amino-3, 6-dioxaoctanoic acid
  • HY-136263

    PARP Cancer
    Biotin-NAD + is a PARylation donor and also a substrate of poly (ADP-ribose) polymerase-1 (PARP1). Under the mediation of PARP1, Biotin-NAD + undergoes poly (ADP-ribosyl) ation to generate biotinylated poly (ADP-ribose) polymers. Biotin-NAD + can be used for pull-down assays of PARylated proteins in renal epithelial cells. Biotin-NAD + is applicable to cancer-related research .
    Biotin-NAD+

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