Biotin-NAD+ 

Biotin-NAD⁺ is a PARylation donor and also a substrate of poly (ADP-ribose) polymerase-1 (PARP1). Under the mediation of PARP1, Biotin-NAD⁺ undergoes poly (ADP-ribosyl) ation to generate biotinylated poly (ADP-ribose) polymers. Biotin-NAD⁺ can be used for pull-down assays of PARylated proteins in renal epithelial cells. Biotin-NAD⁺ is applicable to cancer-related research^[1].

Biotin-NAD+ (20 nM; 30 min) acts as the optimal substrate for PARP1-mediated PARylation in this electrochemical detection system, enabling maximum signal generation via avidin-biotin capture of biotinylated PAR polymers and NA-AgNPs^[1].
Biotin-NAD⁺ (20 nM; 30 min) serves as the optimal substrate for PARP1-mediated PARylation in this SPR detection system, enabling maximum capture of biotinylated PAR polymers on the NA-modified gold chip^[1].
Biotin-NAD+ (6 μg; 24-48 h) serves as an effective PARylation donor to pulldown and detect PARylated proteins, including PARP-1, in mpkCCDc14 cells, revealing a dDAVP time-dependent induction of these modified targets^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1045.95

C₃₉H₅₇N₁₁O₁₇P₂S

146385-37-7

O=C(NCCCCCCNC(CCCC[C@H]1[C@](NC2=O)([H])[C@](N2)([H])CS1)=O)CNC3=C4C(N(C=N4)[C@@H]5O[C@H](COP(O)(OP(OC[C@@H]6[C@@H](O)[C@@H](O)[C@H]([N+]7=CC=CC(C(N)=O)=C7)O6)([O-])=O)=O)[C@@H](O)[C@H]5O)=NC=N3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yang S, et al. Electrochemical detection of poly(ADP-ribose) polymerase-1 with silver nanoparticles as signal labels by integrating the advantages of homogeneous reaction with surface-tethered detection. Talanta. 2025;281:126796.  [Content Brief]

  [2]. Jang HJ, et al. Poly(ADP-ribose) polymerase-1 affects vasopressin-mediated AQP2 expression in collecting duct cells of the kidney. Am J Physiol Renal Physiol. 2024;326(1):F69-F85.  [Content Brief]