2. Metabolic Enzyme/Protease
  3. PEPCK
  4. SKF-34288 hydrochloride

SKF-34288 hydrochloride  (Synonyms: 3-Mercaptopicolinic acid hydrochloride; 3-MPA hydrochloride)

Cat. No.: HY-128923 Purity: 98.61%
COA
SDS
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SKF-34288 (3-Mercaptopicolinic acid) hydrochloride is an orally active phosphoenolpyruvate carboxykinase (PEPCK) inhibitor (Ki: 2-9 μM). SKF-34288 hydrochloride is a potent hypoglycemic agent by inhibiting glucose synthesis. SKF-34288 hydrochloride also inhibits Asn metabolism and increases amino acids and amides.

For research use only. We do not sell to patients.

CAS No. : 320386-54-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Customer Review

Based on 8 publication(s) in Google Scholar

SKF-34288 hydrochloride Related Antibodies

Top Publications Citing Use of Products

    SKF-34288 hydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Med. 2025 May 5;222(5):e20240902.  [Abstract]

    BALB/c mice bearing 4T-1 tumors were with or without 3-MPA hydrochloride (SKF-34288 hydrochloride) (100 mg/kg; i.p.; once every 3 days for 7 times), anti–PD-1 antibody, or the combination. The mice were euthanized and examined for tumor growth 30 days after injection, tumor volumes were calculated, and tumors were weighed.

    SKF-34288 hydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Med. 2025 May 5;222(5):e20240902.  [Abstract]

    Representative bioluminescence images from the indicated days after the orthotopical injection of EMT-6 luciferase-expressing cells (1 × 106) into the mammary fat pad of female BALB/c mice treated with control, 3-MPA (SKF-34288 hydrochloride) (100 mg/kg; i.p.; once every 3 days for 7 times), anti–PD-1 antibody, or the combination. The quantification of the bioluminescence imaging is shown (n = 6 per group).

    SKF-34288 hydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Med. 2025 May 5;222(5):e20240902.  [Abstract]

    SKF-34288 hydrochloride (3-MPA) (100 mg/kg; i.p.; once every 3 days for 7 times). IHC analyses of the indicated xenograft tumors with the indicated antibodies. Representative staining images are shown, and the indicated staining scores for the indicated tumor samples were compared. Scale bar, 50 μm.

    SKF-34288 hydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Med. 2025 May 5;222(5):e20240902.  [Abstract]

    BALB/c mice bearing 4T-1 tumors were treated with or without 3-MPA hydrochloride (SKF-34288 hydrochloride) (100 mg/kg; i.p.; once every 3 days for 7 times), anti–PD-1 antibody, or the combination. Intratumoral cells were harvested for scRNA-seq analyses. UMAP analyses of CD45+ immune cells that colored by major cell types, comparison of major CD45+ immune cell proportion, and Ro/e analysis were performed.

    SKF-34288 hydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Jul 26;10(30):eado3141.  [Abstract]

    The Atf3fl/fl or Atf3M−/− hepatic macrophages were treated with 0.25 mM SKF-34288 hydrochloride or vehicle for 24 hours, and FAO was determined (left; n = 5 per group). The Lv-Gfp or Lv-Atf3 hepatic macrophages were treated with 0.25 mM SKF-34288 hydrochloride or vehicle for 24 hours, and FAO was determined (right, n = 4 per group).

    SKF-34288 hydrochloride purchased from MedChemExpress. Usage Cited in: Metabolism. 2024 Jun 13:155940.  [Abstract]

    The pyruvate tolerance test (PTT) in mice treated with norepinephrine (Nor; 1 mg/kg); glycemia (N = 5). The results showed that injection of Nor in normal mice increased glucose output from pyruvate and this effect was abolished by 3Mpa (SKF-34288 hydrochloride) (30 mg/kg; oral gavage; single dose).

    SKF-34288 hydrochloride purchased from MedChemExpress. Usage Cited in: Metabolism. 2024 Jun 13:155940.  [Abstract]

    Treatment with 3Mpa (SKF-34288 hydrochloride) (30 mg/kg; oral gavage; single dose), a PEPCK inhibitor, in mice exposed to cold for 6 h; glycemia (N = 3–5).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    SKF-34288 (3-Mercaptopicolinic acid) hydrochloride is an orally active phosphoenolpyruvate carboxykinase (PEPCK) inhibitor (Ki: 2-9 μM). SKF-34288 hydrochloride is a potent hypoglycemic agent by inhibiting glucose synthesis. SKF-34288 hydrochloride also inhibits Asn metabolism and increases amino acids and amides[1][2].

    IC50 & Target

    Ki: 2-9 μM (PEPCK)[1]
    In Vitro

    SKF-34288 hydrochloride (0.01-1 mM, 48 h) inhibits C2C12 cell proliferation[4].
    SKF-34288 hydrochloride (0.25-1 mM, 0-5 day) induces myogenic differentiation of C2C12 cells[4].
    SKF-34288 hydrochloride (0.25 or 1 mM) reduces mRNA expression of Pck2 and genes associated with serine biosynthesis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SKF-34288 hydrochloride Related Antibodies

    Cell Viability Assay[4]

    Cell Line: C2C12 cell
    Concentration: 0, 0.01, 0.1, 0.25, 0.5, 1 mM
    Incubation Time: 48 h
    Result: Dose-dependently inhibited C2C12 cell proliferation.
    In Vivo

    SKF-34288 hydrochloride (37.5-150mg/kg, p.o.) reduces blood glucose in starved rats[6].
    SKF-34288 hydrochloride (25 mg/kg, s.c.) neutralizes Salbutamol- mediated hyperglycaemia in rabbits[3].
    SKF-34288 hydrochloride (30 and 300 mg/kg, i.p.) reduces Pyruvate-induced blood glucose level in rats[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Starved rats[6]
    Dosage: 37.5, 75 and 150mg/kg
    Administration: Oral administration (p.o.)
    Result: Showed dose-dependent magnitude and duration of the hypoglycaemic effect.
    Molecular Weight

    191.64

    Formula

    C6H6ClNO2S
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=NC=CC=C1S)O.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 5 mg/mL (26.09 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.2181 mL 26.0906 mL 52.1812 mL
    5 mM 1.0436 mL 5.2181 mL 10.4362 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
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    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 3.33 mg/mL (17.38 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.61%

    SDS (393 KB)

    COA (246 KB) HNMR (240 KB) RP-HPLC (248 KB) MS (228 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.2181 mL 26.0906 mL 52.1812 mL 130.4529 mL
    5 mM 1.0436 mL 5.2181 mL 10.4362 mL 26.0906 mL
    10 mM 0.5218 mL 2.6091 mL 5.2181 mL 13.0453 mL
    15 mM 0.3479 mL 1.7394 mL 3.4787 mL 8.6969 mL
    20 mM 0.2609 mL 1.3045 mL 2.6091 mL 6.5226 mL
    25 mM 0.2087 mL 1.0436 mL 2.0872 mL 5.2181 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    SKF-34288 hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SKF-34288320386-54-73-Mercaptopicolinic acid3-MPASKF34288SKF 34288PEPCKPhosphoenolpyruvate CarboxykinaseC2C12glucose synthesishypoglycemicblood glucoseInhibitorinhibitorinhibit

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