SSR-LDs

Guidelines (The following recommended experimental protocols are for guidance only and should be adjusted according to your specific requirements)
1. Stock Solution Preparation
1.1 Solvent: DMSO.
1.2 Recommended concentration: 1 mM.
2. Working Solution Preparation
2.3 Precautions: Adjust the working solution concentration as needed; prepare and use immediately.
3. Staining Procedures
3.1 Adherent cells^[1]: 4T1 cells, 3T3 cells, HeLa cells, HL7702 cells; trypsin digestion is not required for imaging unless specified.
3.1.1 Incubation conditions: Incubate with 10 μM SSR-LDs for 20 min.
3.1.2 Washing steps: Wash 2-3 times with PBS buffer after incubation.
3.2 Tissue samples^[1][2]: Mouse liver tissue; fatty liver tissue; normal liver tissue.
3.2.1 Incubation conditions:
3.2.1.1 Mouse liver tissue: Immerse in 10 μM SSR-LDs for 1 h.
3.2.1.2 Liver tissue: Use 600 nm single-photon excitation during imaging.
3.2.2 Washing steps: Rinse mouse liver tissue with pre-cooled PBS to remove blood before probe incubation.
4. Control Setup
4.1 Normal cell controls: HL7702 cells and 3T3 cells are used in parallel with cancer cell lines (HeLa cells, 4T1 cells).
4.2 Normal tissue/organism controls: Normal mouse liver tissue; normal zebrafish; normal Kunming mice.
4.3 Colocalization control: Lipid droplet tracer (Bodipy 493/503 (HY-W090090) Green).
5. Detection and Analysis
5.1 Instrument types: Confocal fluorescence microscope; in vivo imaging system; fluorescence imaging system.
5.2 Excitation/emission wavelengths:
5.2.1 SSR-LDs: Ex = 580 nm, Em = 610-750 nm (confocal fluorescence microscope); absorption wavelength = 590 nm, emission wavelength = 663 nm (THF system, fluorescence imaging system).
5.2.2 Bodipy 493/503 Green: Ex = 488 nm, Em = 500-550 nm.
5.3 Result analysis:
5.3.1 Fluorescence intensity change: Stronger red fluorescence is observed in low-polarity environments (such as cancer cells, oleic acid-treated cells/zebrafish, diseased/fatty liver tissues) compared to normal samples with higher polarity.
5.3.2 Fluorescence localization: SSR-LDs specifically target intracellular lipid droplets, with a Pearson correlation coefficient > 0.85 when colocalized with Bodipy 493/503 Green (up to > 0.90 after oleic acid stimulation).
5.3.3 Color change: Red fluorescence is emitted in low-polarity environments; as solvent polarity increases, the solution color changes from red to purple.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.