Swertiajaponin
Based on 1 Customer Validation
Swertiajaponin is a tyrosinase inhibitor, forms multiple hydrogen bonds and hydrophobic interactions with the binding pocket of tyrosinase, with an IC50 of 43.47 μM. Swertiajaponin also inhibits oxidative stress-mediated MAPK/MITF signaling, leading to decrease in tyrosinase protein level. Swertiajaponin suppresses melanin accumulation and exhibits strong anti-oxidative activity.
For research use only. We do not sell to patients.
- Purity: 98.53%
- CAS No.: 6980-25-2
- Formula: C22H22O11
- Molecular Weight:462.40
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
IC50: 43.47 μM (tyrosinase)[1]
Swertiajaponin (2-20 μM, 48 h) shows no cytotoxicity in vitro[1].
Swertiajaponin inhibits melanogenesis in human skin model (10-20 μM, 5 days) and UVB or αMSH-induced melanogenesis in B16F10 cells (2-10 μM, 49 h) in dose-dependent manner[1].
Swertiajaponin (2-10 μM, pretreated for 1 h and then UVB exposure for 30 min) decreases ROS and ONOO- in concentration-dependent manner[1].
Swertiajaponin (2-10 μM, 7 or 49 h) inhibits UVB-induced MAPK activation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HaCat (human keratinocyte), B16F10 (mouse melanoma), and Hs27 (human fibroblast)
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Concentration:2, 5, 10, 20 μM
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Incubation Time:48 h
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Result:Showed no cytotoxicity in these cell lines.
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Cell Line:B16F10
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Concentration:2, 5, 10 μM
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Incubation Time:7 or 49 h (pretreated for 1 h and the cells were stimulated with UVB (10 mJ) for additional 6 h for MAPKs or 48 h)
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Result:Decreased phosphorylated microphthalmia-associated transcription factor (MITF) at 10 μM. Reduced UVB-mediated increase in tyrosinase protein level. Decreased the protein levels of MAPKs at 10 μM.
Chemical Information
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CAS No. 6980-25-2
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Appearance Solid
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Molecular Weight 462.40
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Formula C22H22O11
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Color Light yellow to yellow
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SMILES
O=C1C=C(C2=CC=C(O)C(O)=C2)OC3=CC(OC)=C([C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)C(O)=C13
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (108.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.70 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.70 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1626 mL | 10.8131 mL | 21.6263 mL | 54.0657 mL |
| 5 mM | 0.4325 mL | 2.1626 mL | 4.3253 mL | 10.8131 mL | |
| 10 mM | 0.2163 mL | 1.0813 mL | 2.1626 mL | 5.4066 mL | |
| 15 mM | 0.1442 mL | 0.7209 mL | 1.4418 mL | 3.6044 mL | |
| 20 mM | 0.1081 mL | 0.5407 mL | 1.0813 mL | 2.7033 mL | |
| 25 mM | 0.0865 mL | 0.4325 mL | 0.8651 mL | 2.1626 mL | |
| 30 mM | 0.0721 mL | 0.3604 mL | 0.7209 mL | 1.8022 mL | |
| 40 mM | 0.0541 mL | 0.2703 mL | 0.5407 mL | 1.3516 mL | |
| 50 mM | 0.0433 mL | 0.2163 mL | 0.4325 mL | 1.0813 mL | |
| 60 mM | 0.0360 mL | 0.1802 mL | 0.3604 mL | 0.9011 mL | |
| 80 mM | 0.0270 mL | 0.1352 mL | 0.2703 mL | 0.6758 mL | |
| 100 mM | 0.0216 mL | 0.1081 mL | 0.2163 mL | 0.5407 mL |
Swertiajaponin Related Classifications
HY-N2204 Related Classifications
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