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Cat. No.: HY-W319295

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TEMPOL-H

TEMPOL-H is the reduced hydroxylamine form of the stable nitroxide radical Tempol (HY-100561), and serves as the active metabolite of OT-551 (HY-W556870). TEMPOL-H acts as a potent antioxidant and free radical scavenger. TEMPOL-H protects retinal pigment epithelial cells from acute light-induced damage, and protects mice from lethal whole-body radiation. TEMPOL-H inhibits lens opacification in mice, prevents glutathione loss in rat lenses under stress, blocks protein leakage in rhesus monkey lenses under stress, and maintains the ³H-choline accumulation capacity of the lens. TEMPOL-H can be used in studies related to light-induced retinal pigment epithelial degeneration and age-related cataract.

For research use only. We do not sell to patients.

CAS No.: 3637-10-3
Formula: C₉H₁₉NO₂
Molecular Weight:173.25

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

TEMPOL-H Chemical Structure

CAS No.: 3637-10-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
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- In Vitro
- In Vivo
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Biological Activity

Description

In Vitro

TEMPOL-H inhibits photo-oxidative damage induced by short-wavelength light in lipofuscin fluorophore-loaded retinal pigment epithelial cells in vitro^[1].
Tempol-H (4 mM; 18-20 h) significantly preserves ³H-choline accumulation in organ-cultured Sprague Dawley rat lenses damaged by induction with 250 μM H₂O₂^[3].
Tempol-H (4 mM; 24 h) inhibits 1.0 mM H₂O₂-induced lens opacification in Sprague Dawley rat organ cultures maintained for 24 hours^[3].
Tempol-H (4 mM; 18 h) prevents glutathione loss in lenses from Sprague Dawley rats treated with 1.5 mM H₂O₂ during 18 h organ culture^[3].
Tempol-H (4 mM; 5 h) inhibits oxidative aggregation of water-soluble bovine lens crystallins treated with flavin mononucleotide (FMN) and visible light^[3].
Tempol-H (1-4 mM; 9 days) inhibits xylose (HY-N0537)-induced opacification of rhesus monkey lenses during 9 days of organ culture^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

TEMPOL-H (100 mg/kg; i.p.; single administration 30 min before light exposure) protects RPE cells in the inferior retina of Sprague-Dawley rats against acute light-induced degeneration^[1].
Tempol-H (325 mg/kg; i.p.; single administration; 5-10 minutes prior to irradiation) acts as an in vivo radioprotector in female C3H mice against lethality induced by whole-body irradiation, with a dose modification factor of 1.3^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley (6-7 weeks of age; light-induced retinal pigment epithelium degeneration model)^[1]
Dosage:

100 mg/kg
Administration:

i.p.; single dose 30 minutes prior to light exposure
Result:

Showed no significant difference in retinal pigment epithelium cell nuclei count between light-exposed inferior hemisphere eyes and non-light-exposed control eyes.
Demonstrated a significantly higher retinal pigment epithelium damage index than 100 mg/kg OT-551 in the superior hemisphere of light-exposed eyes.

Animal Model:

C3H mice (female, 60-80 days old, 20-30 g, whole-body γ-irradiation with ¹³⁷Cs source at 1 Gy/min dose rate)^[2]
Dosage:

325 mg/kg
Administration:

i.p.; single dose; 5-10 minutes pre-irradiation
Result:

Provided radioprotection against whole-body radiation-induced lethality at 30 days: near-complete survival was observed at 8 Gy and 9 Gy, with protection seen up to 13 Gy.
Achieved a dose modification factor (DMF) of 1.3.

Chemical Information

CAS No. 3637-10-3
Molecular Weight 173.25
Formula C₉H₁₉NO₂
SMILES

OC1CC(C)(C)N(O)C(C)(C)C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Tanito M, et al. Protection of retinal pigment epithelium by OT-551 and its metabolite TEMPOL-H against light-induced damage in rats. Experimental eye research. 2010 Jul;91(1):111-4. [Content Brief]

[2]. Hahn SM, et al. Evaluation of the hydroxylamine Tempol-H as an in vivo radioprotector. Free radical biology & medicine. 2000 Mar 15;28(6):953-8.

[3]. Zigler JS, et al. Tempol-H inhibits opacification of lenses in organ culture. Free radical biology & medicine. 2003 Nov 15;35(10):1194-202.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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