Tetradifon

Cat. No.: HY-119725 Purity: 99.78%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Tetradifon is a broad-spectrum organochlorine insecticide and an inhibitor of the mitochondrial oligomycin sensitivity conferring protein (OSCP), which can be used to control a variety of mites. Tetradifon inhibits energy-related activities such as ADP-stimulated respiration, DNP and Mg²⁺-stimulated ATPase, with an IC₅₀ of 4.5-27 nmoL/mg mitochondrial protein. Tetradifon exerts oligomycin-like activity by inhibiting the oxidative phosphorylation process, inducing oxidative stress and interfering with bone metabolism. Tetradifon is currently mainly used in the research of mitochondrial function regulation, bone remodeling mechanism and nephrotoxicity of environmental pollutants.

For research use only. We do not sell to patients.

Tetradifon Chemical Structure

CAS No. : 116-29-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Other Forms of Tetradifon:

Tetradifon (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Mite

In Vitro

Tetradifon has a biological mechanism similar to Oligomycin (HY-N6782) and can be reversed by DNP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tetradifon (24.3 mg/kg; oral, 90 days) causes increased trabecular thickness and elevated serum alkaline phosphatase in female rats, and induces glomerular sclerosis and renal interstitial fibrosis in rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Wistar rats (190 g, 8-9 weeks, n=12/group)^[3]
Dosage:	2430 mg/kg cumulative (oral in drinking water, 90 days)
Administration:	Daily oral gavage, 6 and 12 weeks
Result:	At 12 weeks, increased creatinine/urea levels by 24%/43%, increased glomerular sclerosis rate to 22%, and increased interstitial fibrosis index by 200%. Decreased renal SOD/GPx activities by 32%/17%, and decreased vitamin C content by 31%.

Molecular Weight

356.05

Formula

C₁₂H₆Cl₄O₂S

CAS No.

116-29-0

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=C(C=C(C(Cl)=C1)Cl)Cl)(C2=CC=C(C=C2)Cl)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (140.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8086 mL	14.0430 mL	28.0859 mL
5 mM	0.5617 mL	2.8086 mL	5.6172 mL
10 mM	0.2809 mL	1.4043 mL	2.8086 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.78%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (252 KB) HNMR (255 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Desaiah D, et al. Tetradifon (tedion R ): a specific inhibitor of Mg 2+ dependent mitochondrial adenosine triphosphatase activity. Life Sci II. 1972 Apr 8;11(7):389-95. [Content Brief]

[2]. Bustamante E, et al. Tetradifon: an oligomycin-like inhibitor of energy-linked activities of rat liver mitochondria. Biochem Biophys Res Commun. 1973 Mar 17;51(2):292-8. [Content Brief]

[3]. Badraoui R, et al. Effect of subchronic exposure to tetradifon on bone remodelling and metabolism in female rat. C R Biol. 2007 Dec;330(12):897-904. [Content Brief]

[4]. Badraoui R, et al. Nephrotoxic effect of tetradifon in rats: a biochemical and histomorphometric study. Exp Toxicol Pathol. 2012 Sep;64(6):645-50. [Content Brief]

[5]. Fatemeh Ganjeizadeh Rohani, et al. Electropolymerized MIP With MWCNTs on Stir Bar Using Multivariate Optimization for Tetradifon Detection in Date. Pharm Nanotechnol. 2019;7(5):404-417. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8086 mL	14.0430 mL	28.0859 mL	70.2149 mL
	5 mM	0.5617 mL	2.8086 mL	5.6172 mL	14.0430 mL
	10 mM	0.2809 mL	1.4043 mL	2.8086 mL	7.0215 mL
	15 mM	0.1872 mL	0.9362 mL	1.8724 mL	4.6810 mL
	20 mM	0.1404 mL	0.7021 mL	1.4043 mL	3.5107 mL
	25 mM	0.1123 mL	0.5617 mL	1.1234 mL	2.8086 mL
	30 mM	0.0936 mL	0.4681 mL	0.9362 mL	2.3405 mL
	40 mM	0.0702 mL	0.3511 mL	0.7021 mL	1.7554 mL
	50 mM	0.0562 mL	0.2809 mL	0.5617 mL	1.4043 mL
	60 mM	0.0468 mL	0.2340 mL	0.4681 mL	1.1702 mL
	80 mM	0.0351 mL	0.1755 mL	0.3511 mL	0.8777 mL
	100 mM	0.0281 mL	0.1404 mL	0.2809 mL	0.7021 mL

Tetradifon Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetradifon116-29-0Environmental PollutantsInsecticideEnvironmental ContaminantsPesticideorganochlorineinsecticidemitesacaricideInhibitorinhibitorinhibit

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