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Cat. No.: HY-157307A

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TTBK1-IN-4, negative control

TTBK1-IN-4, negative control is a negative control of TTBK1-IN-4 (HY-157307), with IC₅₀ values of 4300 nM and 3600 nM for TTBK1 and TTBK2, respectively. TTBK1-IN-4, negative control lacks intracellular TTBK1/TTBK2 affinity, does not eliminate TTBK2 autophosphorylation, does not recruit IFT88, and does not disrupt ciliogenesis. TTBK1-IN-4, negative control can be used for research on ciliogenesis-related diseases.

For research use only. We do not sell to patients.

Formula: C₁₇H₁₇N₅O
Molecular Weight:307.35

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (282 KB)

TTBK1-IN-4, negative control Chemical Structure

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Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

TTBK1

4.3 μM (IC₅₀)

TTBK2

3.6 μM (IC₅₀)

In Vitro

TTBK1-IN-4, negative control (compound 5), exhibits only weak activity against TTBK1 and TTBK2 in cell-free radioactive enzymatic assays, with IC₅₀ values of 4.3 μM and 3.6 μM, respectively^[1].
TTBK1-IN-4, negative control, shows no intracellular binding of TTBK1 and TTBK2 in the HEK293 NanoBRET assay, with IC₅₀ values >30 μM for both isoforms^[1].
TTBK1-IN-4, negative control (0.5-10 μM; 8 h) does not reduce HEK293/SH-SY5Y cell viability^[1].
TTBK1-IN-4, negative control (10 μM; 4 h) does not eliminate TTBK2 autophosphorylation in hTERT-RPE-1 cells and does not inhibit the recruitment of IFT88 to centrioles in hTERT-RPE-1 cells^[1].
TTBK1-IN-4, negative control (1 μM; 24 h) does not disrupt ciliogenesis in hTERT-RPE-1 cells at 1 μM after 24 h, with approximately 70% of cells still retaining cilia^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HEK293 and SH-SY5Y cells
Concentration:

0.5, 1.0, and 10 μM
Incubation Time:

8 h
Result:

Did not reduce HEK293/SH-SY5Y cell viability.

Western Blot Analysis^[1]

Cell Line:

hTERT-RPE-1 cells
Concentration:

10 μM
Incubation Time:

4 h
Result:

Did not ablate TTBK2 autophosphorylation in hTERT-RPE-1 cells.

Immunofluorescence^[1]

Cell Line:

hTERT-RPE-1 cells
Concentration:

10 μM
Incubation Time:

4 h
Result:

Did not inhibit IFT88 recruitment to centrioles in hTERT-RPE-1 cells.

Immunofluorescence^[1]

Cell Line:

hTERT-RPE-1 cells
Concentration:

1 μM
Incubation Time:

24 h
Result:

Did not disrupt ciliogenesis in hTERT-RPE-1 cells at 1 μM after 24 h, with approximately 70% of cells remaining ciliated.

Chemical Information

Molecular Weight 307.35
Formula C₁₇H₁₇N₅O
SMILES

OCC(C)(C)C#CC1=CC(N(C2=NC(N)=NC=C2)C=C3)=C3C=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Flax R G, et al. Potent and Cell Active 5-Azaindole-based Tau Tubulin Kinase Inhibitors Disrupt Ciliogenesis. J. Med. Chem., 2026.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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