Uralodin B
Uralodin B is a naturally occurring polycyclic polyprenylated acylphloroglucinol (PPAP) and is the C (30)-epimer of Uralodin C. It is found in the aerial parts of Hypericum henryi subsp. uraloides. Uralodin B exhibits cytotoxic activity against SGC7901 and HL-60 tumor cells. It is useful for research related to PPAP natural products and tumor cytotoxicity.
For research use only. We do not sell to patients.
- CAS No.: 1253206-38-0
- Formula: C38H50O5
- Molecular Weight:586.80
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
21.8 μM
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Cytotoxic activity against human HL-60 promyelocytic leukemia cells assessed by MTT assay after 48 h of continuous exposure.
Cytotoxic activity against human HL-60 promyelocytic leukemia cells assessed by MTT assay after 48 h of continuous exposure.
|
20087990 |
| SGC-7901 | IC50 |
63.7 μM
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Cytotoxic activity against human SGC7901 gastric carcinoma cells assessed by MTT assay after 48 h of continuous exposure.
Cytotoxic activity against human SGC7901 gastric carcinoma cells assessed by MTT assay after 48 h of continuous exposure.
|
20087990 |
| HepG2 | IC50 |
171.0 μM
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Cytotoxic activity against human HepG2 hepatocellular carcinoma cells assessed by MTT assay after 48 h of continuous exposure.
Cytotoxic activity against human HepG2 hepatocellular carcinoma cells assessed by MTT assay after 48 h of continuous exposure.
|
20087990 |
| K562 | IC50 |
171.0 μM
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Cytotoxic activity against human K562 chronic myelogenous leukemia cells assessed by MTT assay after 48 h of continuous exposure.
Cytotoxic activity against human K562 chronic myelogenous leukemia cells assessed by MTT assay after 48 h of continuous exposure.
|
20087990 |
In Vitro
Uralodin B (0.064-40 μM; 48 h) exerts modest cytotoxic effects against HL-60 and SGC7901 cells with IC50 values of 21.8 μM and 63.7 μM, respectively, while showing weak activity against HepG2 and K562 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HepG2 human hepatocellular carcinoma cells, SGC7901 human gastric carcinoma cells, HL-60 human promyelocytic leukemia cells, K562 human chronic myelogenous leukemia cells
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Concentration:0.064, 0.32, 1.6, 8, 40 μM
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Incubation Time:48 h
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Result:Exhibited the most potent cytotoxic activity against HL-60 cells, with an IC50 of 21.8 μM.
Showed modest cytotoxicity against SGC7901 cells, with an IC50 of 63.7 μM.
Showed minimal cytotoxic activity against HepG2 and K562 cell lines, with IC50 values of 171.0 μM for both cell lines.
Chemical Information
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CAS No. 1253206-38-0
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Molecular Weight 586.80
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Formula C38H50O5
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SMILES
O=C(C1=CC=CC=C1)[C@@]23[C@@](C)([C@H](C[C@@](C2=O)(C4=C(C[C@@H](O4)C(C)(O)C)C3=O)C/C=C(C)\C)C/C=C(C)\C)CC/C=C(C)/C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Uralodin B Related Classifications
HY-N21698 Related Classifications
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Keywords
- Uralodin B
- 1253206-38-0
- Endogenous Metabolite
- hepatocellular carcinoma
- myeloid leukemia cancer cells
- SGC7901
- bicyclo[3.3.1]nonane-2,4,9-trione core
- Hypericum henryi subsp. uraloides
- HL-60
- methylated polycyclic polyprenylated acylphloroglucinol
- HepG2
- chronic myeloid leukemia cancer cells
- gastric cancer cells
- Inhibitor
- inhibitor
- inhibit