Wyerone
Wyerone is a furan-acetylene phytoalexin that exhibits antifungal activity and selective toxicity against Gram-positive bacteria. Wyerone is abundantly biosynthesized and accumulated in broad bean tissues following infection by Botrytis, and it inhibits the growth of Gram-positive bacteria. Wyerone is applicable to studies related to plant infections.
For research use only. We do not sell to patients.
- CAS No.: 20079-30-5
- Formula: C15H14O4
- Molecular Weight:258.27
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Wyerone exhibits selective antibacterial activity: it inhibits all tested Gram-positive bacterial strains while showing no activity against any of the tested Gram-negative strains. Furthermore, when Wyerone is applied to assay filter discs using different solvents such as acetone, diethyl ether, or methanol, there is no significant difference in the measured sizes of the inhibition zones against Gram-positive bacteria[1].
Wyerone produces clear inhibition zones against all 5 tested Gram-positive bacteria, and this effect occurs at concentrations no higher than 4 μg per disc. Overall, the area of the inhibition zones shows an increasing trend as the concentration rises from 2 μg per disc to 10 μg per disc[1].
Wyerone (up to 30 μg per disk; 24 h) induces concentration-dependent increases in the area of inhibition zones in Bacillus megaterium cultures, with the maximum effective concentration reaching 30 μg per disk; no additional growth inhibitory effect is observed above this concentration[1].
Wyerone (4 days post-infection): When broad bean leaves are infected by Botrytis fabae, the biosynthesis of wyerone is strongly stimulated, reaching a peak of 45 μg/g on day 4 post-infection[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 20079-30-5
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Molecular Weight 258.27
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Formula C15H14O4
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SMILES
CC/C=C\C#CC(C1=CC=C(/C=C/C(OC)=O)O1)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Wyerone Related Classifications
HY-N21819 Related Classifications
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