Cat. No.: HY-N8423

α-Amyrin

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Based on 1 publication(s) in Google Scholar

α-Amyrin is a pentacyclic triterpenoid compound with oral activity. α-Amyrin activates the ERK and GSK-3β signaling pathways. α-Amyrin can inhibit cancer cells proliferation and induce apoptosis. α-Amyrin shows anti-bacterial and anti-inflammation activity. α-Amyrin can reduce blood glucose level. α-Amyrin can be used for the researches of cancer, infection, inflammation, metabolic disease and neurological disease, such as breast cancer, Streptococcus oralis infection, skin inflammation and diabetes.

For research use only. We do not sell to patients.

Purity: 99.99%
CAS No.: 638-95-9
Formula: C₃₀H₅₀O
Molecular Weight:426.72

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

α-Amyrin Chemical Structure

CAS No.: 638-95-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

GSK-3β

Caspase 3

COX-2

All ERK Isoforms :

All GSK-3 Isoforms :

All Caspase Isoforms :

All COX Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	20.6 μg/mL Compound: alpha-amyrin	Cytotoxicity against human A2780 cells Cytotoxicity against human A2780 cells	[PMID: 15165160]
A549	GI₅₀	10.54 μM Compound: 58	Cytotoxicity against human A549 cells after 48 hrs by WST8 assay Cytotoxicity against human A549 cells after 48 hrs by WST8 assay	[PMID: 20371180]
B16	ED₅₀	>50 μM Compound: 19	Induction of melanogenesis in mouse B16 2F2 cells assessed as intracellular melanin content after 3 days Induction of melanogenesis in mouse B16 2F2 cells assessed as intracellular melanin content after 3 days	[PMID: 12027734]
B16	IC₅₀	50 μM Compound: 19	Growth inhibition of mouse B16 2F2 cells after 3 days Growth inhibition of mouse B16 2F2 cells after 3 days	[PMID: 12027734]
CHO	EC₅₀	>10 μM Compound: Alpha-amyrin	Agonist activity at TGR5 expressed in CHO cells by CRE-driven luciferase reporter gene assay Agonist activity at TGR5 expressed in CHO cells by CRE-driven luciferase reporter gene assay	[PMID: 19911773]
COS-1	EC₅₀	0 μM Compound: Alpha-amyrin	Agonist activity at human FXR expressed in COS1 cells by luciferase reporter gene assay Agonist activity at human FXR expressed in COS1 cells by luciferase reporter gene assay	[PMID: 19911773]
HepG2	IC₅₀	>100 μM Compound: 8	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by neutral red assay Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by neutral red assay	[PMID: 28318944]
HepG2	IC₅₀	220 μM Compound: 8	Cytotoxicity against human Hep G2 cells after 48 hrs by WST-8 assay Cytotoxicity against human Hep G2 cells after 48 hrs by WST-8 assay	[PMID: 15730243]
J774	IC₅₀	128.5 μM Compound: 1	Cytotoxicity against mouse J774 cells by alamar blue assay Cytotoxicity against mouse J774 cells by alamar blue assay	[PMID: 17637068]
KB	IC₅₀	>100 μM Compound: 8	Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by neutral red assay Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by neutral red assay	[PMID: 28318944]
Lu1	IC₅₀	>100 μM Compound: 8	Cytotoxicity against human Lu1 cells assessed as growth inhibition after 72 hrs by neutral red assay Cytotoxicity against human Lu1 cells assessed as growth inhibition after 72 hrs by neutral red assay	[PMID: 28318944]
MCF7	IC₅₀	>100 μM Compound: 8	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by neutral red assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by neutral red assay	[PMID: 28318944]
PANC-1	GI₅₀	12.8 μM Compound: 58	Cytotoxicity against human PANC1 cells after 48 hrs by WST8 assay Cytotoxicity against human PANC1 cells after 48 hrs by WST8 assay	[PMID: 20371180]
PC-3	GI₅₀	7.66 μM Compound: 58	Cytotoxicity against human PC3 cells after 48 hrs by WST8 assay Cytotoxicity against human PC3 cells after 48 hrs by WST8 assay	[PMID: 20371180]
SH-SY5Y	EC₅₀	>50 μM Compound: 37	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 1-methyl-4-phenylpyridinium ion-induced cell death after 48 hrs by MTT assay Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 1-methyl-4-phenylpyridinium ion-induced cell death after 48 hrs by MTT assay	[PMID: 27420919]
SH-SY5Y	EC₅₀	13.3 μM Compound: 37	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in oxygen glucose deprivation-induced cell death incubated for 16 hrs under hypoxic condition followed by incubation for 24 hrs under normoxic condition by MTT assay Neuroprotective activity in human SH-SY5Y cells assessed as reduction in oxygen glucose deprivation-induced cell death incubated for 16 hrs under hypoxic condition followed by incubation for 24 hrs under normoxic condition by MTT assay	[PMID: 27420919]
WI-38	IC₅₀	12.7 μM Compound: 8	Cytotoxicity against human WI 38 cells after 48 hrs by WST-8 assay Cytotoxicity against human WI 38 cells after 48 hrs by WST-8 assay	[PMID: 15730243]
WI-38 VA13	IC₅₀	>235 μM Compound: 8	Cytotoxicity against human VA13 cells after 48 hrs by WST-8 assay Cytotoxicity against human VA13 cells after 48 hrs by WST-8 assay	[PMID: 15730243]

In Vitro

α-Amyrin (10 μM, 24 h) induces AMPK and PPARδ/ γ-mediated GLUT4 translocation in C2C12 myoblasts^[1].
α-Amyrin (acetate) (2.5-10 μg/mL, 0-72 h) inhibits proliferation and colony formation in MDA-MB-231 cells^[6].
α-Amyrin (acetate) (5-10 μg/mL, 24 h) induces apoptosis and increases caspase-3 activity in MDA-MB-231 cells^[6].
α-Amyrin (acetate) (10 μg/mL, 24 h) promotes the cleavage of Poly polymerase, increased E-cadherin, and inhibits vimentin and phosphorylated extracellular signal-regulated activation in MDA-MB-231 cells^[6].
α-Amyrin (10-1000 μg/mL, 24 h) reduces percentage of viability of Streptococcus salivarius, Streptococcus sanguinis and Streptococcus oralis^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

C2C12 cells
Concentration:

10 μM
Incubation Time:

24 h
Result:

Increased the mRNA and protein expression of PPARs, GLUT4 and FATP.

Apoptosis Analysis^[2]

Cell Line:

MDA-MB-231 cells
Concentration:

5 and 10 μg/mL
Incubation Time:

24 h
Result:

Increased apoptotic cells.
Increased caspase-3 activity.

In Vivo

α-Amyrin (50-200 mg/kg, p.o., once daily for 42 days) can reduce the survival rate of rats with metabolic syndrome induced by high fructose diet^[2].
α-Amyrin (2-4 mg/kg, p.o.) improves cognitive dysfunction caused by low cholinergic neurotransmission in mice by activating ERK and GSK-3β signaling pathways^[3].
α-Amyrin (acetate) (50 mg/kg, p.o., daily for 1-10 days) reduces the blood glucose level in diabetic rats^[4].
α-Amyrin (0.1-1.0 mg/ear, topical application) inhibits inflammation in 12-O-tetradecanoylphorbol-acetate (TPA)-induced skin inflammation of mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

High-fructose diet (HFD)-induced metabolic syndrome in rats^[2]
Dosage:

50, 100 and 200 mg/kg
Administration:

Orally administration
Result:

Decreased systolic blood pressure, plasma glucose, total cholesterol, and plasma triglycerides.
Attenuated hepatic oxidative stress as well as micro- and macrovesicular fatty changes in hepatocytes caused by HFD.

Animal Model:

Scopolamine-Induced Memory Impairment Mice^[3]
Dosage:

2 and 4 mg/kg
Administration:

Orally administration
Result:

Increased the expression levels of phosphorylated extracellular signal-regulated kinase 1/2 (pERK) and phosphorylated glycogen synthase kinase-3β (pGSK-3β).

Animal Model:

12-O-tetradecanoylphorbol-acetate (TPA)-induced skin inflammation of mice^[5]
Dosage:

0.1, 0.3 and 1.0 mg/ear, topical application
Administration:

Topically application
Result:

Inhibited skin inflammation.
Reduced COX-2 levels and activation of NF-κB and MAPKs.

Chemical Information

CAS No. 638-95-9
Appearance Solid
Molecular Weight 426.72
Formula C₃₀H₅₀O
Color White to off-white
SMILES

C[C@]12[C@]3(C([C@@]4([H])[C@@](CC3)(CC[C@H]([C@@H]4C)C)C)=CC[C@]1([H])[C@@]5([C@@](C(C)([C@H](CC5)O)C)([H])CC2)C)C
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Trop Med Infect Dis

Lupeol Acetate and α-Amyrin Terpenes Activity against Trypanosoma cruzi: Insights into Toxicity and Potential Mechanisms of Action. [Abstract]

2023 May 3;8(5):263. PMID: 37235311

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (11.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Methanol : 1 mg/mL (2.34 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3435 mL

11.7173 mL

23.4346 mL

5 mM

0.4687 mL

2.3435 mL

4.6869 mL

10 mM

0.2343 mL

1.1717 mL

2.3435 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Giacoman-Martínez A, et al. α-Amyrin induces GLUT4 translocation mediated by AMPK and PPARδ/γ in C2C12 myoblasts. Can J Physiol Pharmacol. 2021 Sep;99(9):935-942. [Content Brief]

[2]. Prabhakar P, et al. α-Amyrin attenuates high fructose diet-induced metabolic syndrome in rats. Appl Physiol Nutr Metab. 2017 Jan;42(1):23-32. [Content Brief]

[3]. Park SJ, et al. Amyrin attenuates scopolamine-induced cognitive impairment in mice. Biol Pharm Bull. 2014;37(7):1207-13. [Content Brief]

[4]. Singh AB, et al. Antihyperglycaemic activity of alpha-amyrin acetate in rats and db/db mice. Nat Prod Res. 2009;23(9):876-82. [Content Brief]

[5]. Medeiros R, et al. Mechanisms underlying the inhibitory actions of the pentacyclic triterpene alpha-amyrin in the mouse skin inflammation induced by phorbol ester 12-O-tetradecanoylphorbol-13-acetate. Eur J Pharmacol. 2007 Mar 22;559(2-3):227-35. [Content Brief]

[6]. Kevine Kamga Silihe, Purification of α-amyrin-acetate and phenylpropanoïd compounds from Ficus umbellata Vahl (Moraceae) stem bark and evaluation of their anti-breast cancer potential, South African Journal of Botany, 177 (2025) 445-456.

[7]. Díaz-Ruiz G, et al. Growth inhibition of Streptococcus from the oral cavity by α-amyrin esters. Molecules. 2012 Oct 25;17(11):12603-11. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Methanol / DMSO	1 mM	2.3435 mL	11.7173 mL	23.4346 mL	58.5864 mL
DMSO	5 mM	0.4687 mL	2.3435 mL	4.6869 mL	11.7173 mL
DMSO	10 mM	0.2343 mL	1.1717 mL	2.3435 mL	5.8586 mL

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α-Amyrin

Apoptosis Compound Library

Immunology/Inflammation Compound Library

MAPK Compound Library

PI3K/Akt/mTOR Compound Library

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Publications Citing Use of MedChemExpress (MCE) α-Amyrin

All ERK Isoforms

All GSK-3 Isoforms

All Caspase Isoforms

All COX Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

α-Amyrin Related Classifications

Cat. No.: HY-N8423