β-Elemene
Based on 8 publication(s) in Google Scholar
β-Elemene ((-)-β-Elemene; Levo-β-elemene) is isolated from natural plant Curcuma aromatica with an antitumor activity. β-Elemene can induce cell apoptosis.
For research use only. We do not sell to patients.
- Purity : 99.62%
- CAS No.: 515-13-9
- Formula: C15H24
- Molecular Weight:204.35
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-13259
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Publications Citing Use of MedChemExpress (MCE) β-Elemene
More- Adv Sci (Weinh). 2026 May 27:e21445. [Abstract]
- Phytomedicine. 2026 Jul:156:158242. [Abstract]
- Antioxidants. 2024 Dec 9;13(12):1499. [Abstract]
- Sci Rep. 2023 Jul 27;13(1):12160. [Abstract]
- IUBMB Life. 2022 Jun;74(6):508-518. [Abstract]
- BMC Pulm Med. 2025 Feb 28;25(1):97. [Abstract]
- Biochim Biophys Acta Gen Subj. 2026 Mar;1870(3):130901. [Abstract]
- Heliyon. 2024 May 18;10(10):e31537. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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Cell Proliferation/Viability Assay
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RT-PCR
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WB
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
227 μM
Compound: 1
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Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
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[PMID: 36240626] |
| HCT-116 | IC50 |
781.9 μM
Compound: 1
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Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
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[PMID: 36240626] |
| HeLa | IC50 |
213.51 μM
Compound: beta-Elemene
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Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay
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[PMID: 28463784] |
| HeLa | IC50 |
236.2 μM
Compound: beta-elemene
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Antiproliferative activity against human HeLa cells after 24 hrs by WST-1 assay
Antiproliferative activity against human HeLa cells after 24 hrs by WST-1 assay
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[PMID: 19128976] |
| PC-3 | IC50 |
276.2 μM
Compound: 1
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Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
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[PMID: 36240626] |
| SGC-7901 | IC50 |
236.27 μM
Compound: beta-Elemene
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Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay
Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay
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[PMID: 28463784] |
| U-251 | IC50 |
180.8 μM
Compound: 1
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Antiproliferative activity against human U-251 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
Antiproliferative activity against human U-251 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
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[PMID: 36240626] |
| U-87MG ATCC | IC50 |
179.72 μM
Compound: beta-Elemene
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Antiproliferative activity against human U87 cells after 72 hrs by MTT assay
Antiproliferative activity against human U87 cells after 72 hrs by MTT assay
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[PMID: 28463784] |
| U-87MG ATCC | IC50 |
266.3 μM
Compound: 1
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Antiproliferative activity against human U-87 MG cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
Antiproliferative activity against human U-87 MG cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control
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[PMID: 36240626] |
In Vitro
β-Elemene (0-200 μg/ml; 24 hours) shows IC50 values of 72.8 μg/ml; 47.4 μg/ml; 61.5 μg/ml; 3.661 μg/ml; 68 μg/ml; 72.12 μg/ml; 37.894 μg/ml and 37.703 μg/ml for SV-HUC-1, T24, 5637, TCCSUP, J82,UMUC-3,RT4, and SW780 cells, respectively[1]. β-Elemene (0-75 μg/ml; 24 hours) decreases cell number from 50 μg/ml and is notably decreased at 75 μg/ml, induces dose-dependent G1-phase arrest in T24cells and significantly reduces the percentage of cells in the S-phase[1]. β-Elemene (50 μg/ml; 12 hours) downregulates the expression levels of p-STAT3 and the cell cycle-related proteins cyclin D1, CDK4 and CDK6, and upregulates p21 and p27expression in T24 cells [1]. β-Elemene (50 μg/ml; 24 hours) enhances cisplatin-induced apoptosis by activating the ROS-AMPK signaling pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:T24, 5637, TCCSUP, J82, UM-UC-3, RT4 and SW780 cells
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Concentration:0, 6.25, 12.5, 25, 50, 100, 150 and 200 µg/ml
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Incubation Time:24 hours
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Result:Inhibited the proliferation of RT4, SW780, J82, UMUC-3, TCCSUP, 5637 and T24 human bladder cancer cells and SV-HUC-1 human urothelial cells.
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Cell Line:T24 and 5637 cells
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Concentration:0, 25, 50 and 75 µg/ml
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Incubation Time:24 hours
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Result:Induced the percentage of cells in the S-phase as a dose-dependent manner.
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Cell Line:T24 cells
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Concentration:50 µg/ml
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Incubation Time:24 hours
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Result:Downregulated p-STAT3 and cell cycle-related proteins.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 515-13-9
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Appearance Liquid (Density: 0.862 g/cm3)
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Molecular Weight 204.35
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Formula C15H24
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Color Colorless to light yellow
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SMILES
CC([C@H]1[C@@](C)(CC[C@@H](C(C)=C)C1)C=C)=C
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Synonyms
(-)-β-Elemene; Levo-β-elemene
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (8)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
β-Elemene Rescues Radiation-Induced Enteritis by Orchestrating a Host-Microbiome Circuit That Fuels Epigenetic DNA Repair. [Abstract]2026 May 27:e21445. PMID: 42201651 -
Phytomedicine
Targeting UBE2T by β-elemene inhibits prostate cancer stem cells and bone metastasis by blocking the TRIM28/pGSK3β/β-catenin signaling. [Abstract]2026 Jul:156:158242. PMID: 42070337 -
Antioxidants
The Novel Elemene Derivative, OMe-Ph-Elemene, Attenuates Oxidative Phosphorylation and Facilitates Apoptosis by Inducing Intracellular Reactive Oxygen Species. [Abstract]2024 Dec 9;13(12):1499. PMID: 39765827 -
Sci Rep
The pharmacological mechanism of β-elemene in the treatment of esophageal cancer revealed by network pharmacology and experimental verification. [Abstract]2023 Jul 27;13(1):12160. PMID: 37500660
β-Elemene purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jul 27;13(1):12160. [Abstract]
Effects of β-elemene (0-400 μM, 0-72 h) on cell viability and mRNA expression of key targets in ECA-109 cells. Here showed the results of cell viability experiments.
β-Elemene purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jul 27;13(1):12160. [Abstract]
Effects of β-elemene (0-400 μM) on cell viability and mRNA expression of key targets in ECA-109 cells. Here showed the results of the effects of β-elemene on STAT3 mRNA expressions in ECA-109 cells.
β-Elemene purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jul 27;13(1):12160. [Abstract]
Effects of β-elemene (0-200 μM) on protein expression levels of key targets in ECA-109 cells. Here showed the results of the protein expression levels of STAT3, EGFR, BCL2L1, and CASP9 analyzed by western blot.
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IUBMB Life
β-Elemene induces apoptosis by activating the P53 pathway in human hypertrophic scar fibroblasts. [Abstract]2022 Jun;74(6):508-518. PMID: 35294085 -
BMC Pulm Med
β-elemene inhibits tumor-promoting in small cell lung cancer by affecting M2 macrophages and TGF-β. [Abstract]2025 Feb 28;25(1):97. PMID: 40022030 -
Biochim Biophys Acta Gen Subj
Targeting the IGF1/Twist1 axis: A novel mechanism for β-elemene-induced anoikis and EMT inhibition in breast cancer cells. [Abstract]2026 Mar;1870(3):130901. PMID: 41490592 -
Heliyon
β-elemene alleviates esophageal fibrosis after endoscopic submucosal dissection via the FAP-mediated PTEN-PI3K/AKT signaling pathway. [Abstract]2024 May 18;10(10):e31537. PMID: 38807882
β-Elemene purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 18;10(10):e31537. [Abstract]
CCK-8 to assess the proliferation of PHEGFs after β-elemene (0-640=-640Μm, 0-72 h) intervention.
β-Elemene purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 18;10(10):e31537. [Abstract]
β-elemene (480 μM) suppressed the proliferation and promoted the apoptosis of PHEGFs. Flod change of FAP, p-PTEN, p-PI3K, and p-AKT.
Solvent & Solubility
In Vitro:
Ethanol : 50 mg/mL (244.68 mM; Need ultrasonic)
DMSO : 50 mg/mL (244.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
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Subcutaneous Cell-Line-Derived Xenograft
Subcutaneous cell-line-derived xenograft (CDX) models are established by implanting cultured human cancer cell lines into immunodeficient mice, where the injected cells form localized tumors that can be monitored in vivo as a measure of tumorigenic potential, growth kinetics, and treatment response. These models are widely used in oncology research because they allow reproducible tumor formation and enable comparative assessment of tumor growth between different cell lines or genetic manipulations in a controlled in vivo microenvironment. Subcutaneous implantation of cancer cells in immunodeficient mice is a standard approach for evaluating tumor growth behavior and therapeutic response across multiple cancer types, including prostate, esophageal, pancreatic, and colon cancer models.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Ross, S.A., and ElSohly, M.A. The volatile oil composition of fresh and air-dried buds of Cannabis sativa. J. Nat. Prod. 59(1), 49-51 (1996). [Content Brief]
[2]. Gan D, et al. β-elemene enhances cisplatin-induced apoptosis in bladder cancer cells through the ROS-AMPK signaling pathway.Oncol Lett. 2020 Jan;19(1):291-300. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| Ethanol / DMSO | 1 mM | 4.8936 mL | 24.4678 mL | 48.9356 mL | 122.3391 mL |
| 5 mM | 0.9787 mL | 4.8936 mL | 9.7871 mL | 24.4678 mL | |
| 10 mM | 0.4894 mL | 2.4468 mL | 4.8936 mL | 12.2339 mL | |
| 15 mM | 0.3262 mL | 1.6312 mL | 3.2624 mL | 8.1559 mL | |
| 20 mM | 0.2447 mL | 1.2234 mL | 2.4468 mL | 6.1170 mL | |
| 25 mM | 0.1957 mL | 0.9787 mL | 1.9574 mL | 4.8936 mL | |
| 30 mM | 0.1631 mL | 0.8156 mL | 1.6312 mL | 4.0780 mL | |
| 40 mM | 0.1223 mL | 0.6117 mL | 1.2234 mL | 3.0585 mL | |
| 50 mM | 0.0979 mL | 0.4894 mL | 0.9787 mL | 2.4468 mL | |
| 60 mM | 0.0816 mL | 0.4078 mL | 0.8156 mL | 2.0390 mL | |
| 80 mM | 0.0612 mL | 0.3058 mL | 0.6117 mL | 1.5292 mL | |
| 100 mM | 0.0489 mL | 0.2447 mL | 0.4894 mL | 1.2234 mL |
β-Elemene Related Classifications
HY-107324 Related Classifications
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