β-Galactosidase, E. coli
Based on 1 Customer Validation
β-Galactosidase, E. coli (EC 3.2.1.23; GAL) is a glycoside hydrolase that hydrolyzes the β-glycosidic bonds formed between galactose and its organic moieties. β-Galactosidase, E. coli can hydrolyze lactose to form glucose and galactose, and enter glycolysis; it can also catalyze the transgalactosylation of lactose into allolactose; allolactose can be cracked into monosaccharides.
For research use only. We do not sell to patients.
- CAS No.: 9031-11-2
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Inhibitors: Co2+, Cu2+, Ni2+, Zn2+, Proclin
pH range: Effective pH range 3.0-8.0, Optimum pH range 3.5-5.0
Temperature range: Effective temperature range 55°C-65°C, Optimum temperature range 55°C-60°C
Instructions
Enzyme solutions are susceptible to oxidation and should be prepared fresh. Oxidation can be minimized by preparing solutions in 50 mM Tris HCl, pH 7.3, 5- 10 mM β-mercaptoethanol, and 10 mM MgCl2.
Note: Thiol reducing agents should only be used in the presence of magnesium chloride, as β-mercaptoethanol will have an inhibitory effect when magnesium chloride is not present.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.2.1.23
≥50U/mg soild
One unit defined as the amount of enzyme that causes the formation of one micromole of ONP per min at pH 7.3 at 37℃.
Chemical Information
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CAS No. 9031-11-2
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Appearance Solid
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Color White to light yellow
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SMILES
[b-Galactosidase, E. coli]
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Synonyms
EC 3.2.1.23; GAL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
β-Galactosidase, E. coli Related Classifications
HY-P2869 Related Classifications
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