1,3-Diphenylurea
Based on 1 Customer Validation
1,3-Diphenylurea is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 102-07-8
- Formula: C13H12N2O
- Molecular Weight:212.25
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero | CC50 |
351.62 μM
Compound: 13
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Cytotoxicity against african green monkey Vero cells after 2 days by resazurin assay
Cytotoxicity against african green monkey Vero cells after 2 days by resazurin assay
|
[PMID: 25147607] |
1,3-Diphenylurea is a cytokinin. It increases fresh weight of tobacco plant tissue when used at concentrations ranging from 1 to 16 mg/L in growth media.1 1,3-Diphenylurea (32 and 100 μM) increases the growth of cultured P. lunatus callus tissue.2 Exogenous 1,3-diphenylurea (32 and 100 μM) added during first-passage culture induces second-passage cytokinin autonomy in cultured P. lunatus tissue.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 102-07-8
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Appearance Solid
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Molecular Weight 212.25
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Formula C13H12N2O
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Color White to off-white
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SMILES
O=C(NC1=CC=CC=C1)NC2=CC=CC=C2
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Synonyms
sym-Diphenylurea
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (471.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Dyson WH, et al. Short term metabolism of urea and purine cytokinins. Plant Physiol. 1972 Apr;49(4):506-13. [Content Brief]
[2]. Mok MC, et al. Induction of cytokinin autonomy by N,N'-diphenylurea in tissue cultures of Phaseolus lunatus L. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3880-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.7114 mL | 23.5571 mL | 47.1143 mL | 117.7856 mL |
| 5 mM | 0.9423 mL | 4.7114 mL | 9.4229 mL | 23.5571 mL | |
| 10 mM | 0.4711 mL | 2.3557 mL | 4.7114 mL | 11.7786 mL | |
| 15 mM | 0.3141 mL | 1.5705 mL | 3.1410 mL | 7.8524 mL | |
| 20 mM | 0.2356 mL | 1.1779 mL | 2.3557 mL | 5.8893 mL | |
| 25 mM | 0.1885 mL | 0.9423 mL | 1.8846 mL | 4.7114 mL | |
| 30 mM | 0.1570 mL | 0.7852 mL | 1.5705 mL | 3.9262 mL | |
| 40 mM | 0.1178 mL | 0.5889 mL | 1.1779 mL | 2.9446 mL | |
| 50 mM | 0.0942 mL | 0.4711 mL | 0.9423 mL | 2.3557 mL | |
| 60 mM | 0.0785 mL | 0.3926 mL | 0.7852 mL | 1.9631 mL | |
| 80 mM | 0.0589 mL | 0.2945 mL | 0.5889 mL | 1.4723 mL | |
| 100 mM | 0.0471 mL | 0.2356 mL | 0.4711 mL | 1.1779 mL |