1-Methyl-4-nitroimidazole
1-Methyl-4-nitroimidazole is an antibacterial agent. 1-Methyl-4-nitroimidazole is reduced by bacterial nitroreductases to form toxic derivatives, which cause DNA damage, inhibit bacterial nucleic acid synthesis, generate toxic superoxides through futile cycling of reduced radical anions, and induce bacterial cell death. 1-Methyl-4-nitroimidazole exhibits moderate in vitro activity against bacteria and fungal. 1-Methyl-4-nitroimidazole can be used in the research of skin infections, purulent infections and urinary tract infections.
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 3034-41-1
- Formula: C4H5N3O2
- Molecular Weight:127.10
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Anti-Infection Compound Library
HY-L002
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Antifungal Compound Library
HY-L048
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Antibacterial Compound Library
HY-L049
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Human Metabolite Library
HY-L123
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MCE Bioactive Compound Library
HY-L001V
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
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MCE Kinase Hinge Binder Fragment Library
HY-L907
Biological Activity
1-Methyl-4-nitroimidazole (1-methyl-4-nitro-1H-imidazole) (24-48 h) exhibits moderate activity against the tested Gram-positive bacteria, Gram-negative bacteria and fungal isolates, with a maximum inhibition zone of 18 mm against Staphylococcus aureus AB 188 and 18 mm against *Candida tropicalis*[1].
1-Methyl-4-nitroimidazole (100-300 μg/mL; 24 h) inhibits the growth of tested Gram-positive bacteria, Gram-negative bacteria and fungal isolates, with an MIC range of 100-300 μg/mL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3034-41-1
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Appearance Solid
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Molecular Weight 127.10
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Formula C4H5N3O2
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SMILES
CN1C=NC([N+]([O-])=O)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (786.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (39.34 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (39.34 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 7.8678 mL | 39.3391 mL | 78.6782 mL | 196.6955 mL |
| 5 mM | 1.5736 mL | 7.8678 mL | 15.7356 mL | 39.3391 mL | |
| 10 mM | 0.7868 mL | 3.9339 mL | 7.8678 mL | 19.6696 mL | |
| 15 mM | 0.5245 mL | 2.6226 mL | 5.2452 mL | 13.1130 mL | |
| 20 mM | 0.3934 mL | 1.9670 mL | 3.9339 mL | 9.8348 mL | |
| 25 mM | 0.3147 mL | 1.5736 mL | 3.1471 mL | 7.8678 mL | |
| 30 mM | 0.2623 mL | 1.3113 mL | 2.6226 mL | 6.5565 mL | |
| 40 mM | 0.1967 mL | 0.9835 mL | 1.9670 mL | 4.9174 mL | |
| 50 mM | 0.1574 mL | 0.7868 mL | 1.5736 mL | 3.9339 mL | |
| 60 mM | 0.1311 mL | 0.6557 mL | 1.3113 mL | 3.2783 mL | |
| 80 mM | 0.0983 mL | 0.4917 mL | 0.9835 mL | 2.4587 mL | |
| 100 mM | 0.0787 mL | 0.3934 mL | 0.7868 mL | 1.9670 mL |
1-Methyl-4-nitroimidazole Related Classifications
HY-W018143 Related Classifications
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