12-NBD Stearate

12-NBD Stearate (23 µM; 5 min) reflects long-chain fatty acid uptake in 3T3-L1 adipocytes, and its uptake rate significantly decreases to 46.8% and 55% of the control group after disruption of lipid rafts/caveolae by pretreatment with βCD (HY-107201) or Cav^DGV[1].
12-NBD Stearate incubated with BSA (HY-D0842) buffer at different concentrations in 1548 hepatocellular carcinoma cells is taken up linearly by the cells, and its uptake increases with increasing BSA concentration, demonstrating that extracellular binding proteins promote uptake^[2].
12-NBD Stearate (0.5 μM) binds to purified human FABP3, FABP5, and FABP7 with K_d values of 0.18, 0.16, and 0.22 μM, respectively^[3].
12-NBD Stearate (50 µM containing 25 µM BSA; continuously monitored from 12-56 s) exhibits activity in hepatocytes, being rapidly taken up by cells and showing granular cytoplasmic staining, indicating a vesicular uptake pathway^[5].
12-NBD Stearate exhibits maximal and linear initial influx activity in freshly isolated rat hepatocytes, its uptake depends on the presence of BSA, and no similar uptake occurs in erythrocytes^[6].