(1S,2S)-Bortezomib
Based on 1 Customer Validation
(1S,2S)-Bortezomib (Compound Imp-D (1S,2S)) is a diastereomeric impurity of Bortezomib (HY-10227).
For research use only. We do not sell to patients.
- Purity: 97.0%
- CAS No.: 1132709-14-8
- Formula: C19H25BN4O4
- Molecular Weight:384.24
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Ki: 0.6 nM (20S proteasome)[1]
Bortezomib is a reversible and selective proteasome inhibitor that potently inhibits the 20S proteasome by targeting threonine residues (Ki = 0.6 nM). Bortezomib disrupts the cell cycle, induces apoptosis, and inhibits the nuclear factor NF-κB. Bortezomib effectively suppresses the expression of TREM2 in tumor-associated macrophages (TAM). Bortezomib can be used for the research of multiple myeloma.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1132709-14-8
-
Appearance Solid
-
Molecular Weight 384.24
-
Formula C19H25BN4O4
-
Color White to off-white
-
SMILES
OB([C@H](NC([C@@H](NC(C1=NC=CN=C1)=O)CC2=CC=CC=C2)=O)CC(C)C)O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (130.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.51 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.51 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6025 mL | 13.0127 mL | 26.0254 mL | 65.0635 mL |
| 5 mM | 0.5205 mL | 2.6025 mL | 5.2051 mL | 13.0127 mL | |
| 10 mM | 0.2603 mL | 1.3013 mL | 2.6025 mL | 6.5064 mL | |
| 15 mM | 0.1735 mL | 0.8675 mL | 1.7350 mL | 4.3376 mL | |
| 20 mM | 0.1301 mL | 0.6506 mL | 1.3013 mL | 3.2532 mL | |
| 25 mM | 0.1041 mL | 0.5205 mL | 1.0410 mL | 2.6025 mL | |
| 30 mM | 0.0868 mL | 0.4338 mL | 0.8675 mL | 2.1688 mL | |
| 40 mM | 0.0651 mL | 0.3253 mL | 0.6506 mL | 1.6266 mL | |
| 50 mM | 0.0521 mL | 0.2603 mL | 0.5205 mL | 1.3013 mL | |
| 60 mM | 0.0434 mL | 0.2169 mL | 0.4338 mL | 1.0844 mL | |
| 80 mM | 0.0325 mL | 0.1627 mL | 0.3253 mL | 0.8133 mL | |
| 100 mM | 0.0260 mL | 0.1301 mL | 0.2603 mL | 0.6506 mL |
(1S,2S)-Bortezomib Related Classifications
HY-135396 Related Classifications
Powered by Bioz