2,3,5-Trimethylpyrazine
Based on 1 Customer Validation
2,3,5-Trimethylpyrazine is an activator of OR5K1 with an EC50 of 65.03 μM in Hana-3A cells. 2,3,5-Trimethylpyrazine inhibits ZEN biosynthetic gene expression, disrupts cell membrane phospholipid composition, and inhibits mycelial growth. 2,3,5-Trimethylpyrazine inhibits DNA synthesis in the chorioallantoic membrane without disrupting vascular pattern formation. 2,3,5-Trimethylpyrazine inhibits ciliary beating frequency, oocyte pickup, and infundibular smooth muscle contraction in hamster oviduct explants. 2,3,5-Trimethylpyrazine is useful for research related to plant fungal infections.
For research use only. We do not sell to patients.
- Purity : 99.92%
- CAS No.: 14667-55-1
- Formula: C7H10N2
- Molecular Weight:122.17
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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All Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
2,3,5-Trimethylpyrazine (0.1-1000 μM; 2 h) is a potent agonist of OR5K1 expressed in Hana-3A cells, with an EC50 of 65.03 μM[2].
2,3,5-Trimethylpyrazine (0.1-1000 μM; 2 h) synergistically enhances TMP-induced activation of OR5K1 in Hana-3A cells at FA concentrations of 500 μmol/L and 1000 μmol/L[2].
2,3,5-Trimethylpyrazine (0.1-1000 μM; 2 h) synergistically enhances FA-induced activation of OR2W1 in Hana-3A cells, resulting in an approximately 40% increase in signal[2].
2,3,5-Trimethylpyrazine (0.1 pM-10 μM) inhibits ciliary beat frequency in hamster oviductal explants[6].
2,3,5-Trimethylpyrazine (0.1 pM-10 μM) inhibits oocyte pickup rate in hamster oviductal explants[6].
2,3,5-Trimethylpyrazine (0.1 pM-10 μM) inhibits infundibular smooth muscle contraction in hamster oviductal explants[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
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Animal Model:ICA (male, 25-35 g)[3]
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Dosage:4; 6; 8 mg/kg
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Administration:i.p.; single dose; 1 hr prior to MES induction
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Result:Afforded 15% protection at 4 mg/kg, 45% protection at 6 mg/kg, and 93% protection at 8 mg/kg against MES-induced seizures.
Achieved an ED50 of 6.01 mg/kg with 50% protection from seizures.
Demonstrated anticonvulsant activity comparable to diphenylhydantoin (ED50 4.83 mg/kg).
Chemical Information
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CAS No. 14667-55-1
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Appearance Liquid (Density: 0.979 g/cm3)
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Molecular Weight 122.17
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Formula C7H10N2
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Color Colorless to light yellow
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SMILES
CC1=CN=C(C)C(C)=N1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (818.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.46 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.46 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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RT-PCR
Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
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BrdU Incorporation Assay
Bromodeoxyuridine (BrdU) incorporation assay is based on the principle that BrdU, a thymidine analog, is incorporated into newly synthesized DNA during the S phase of the cell cycle, thereby serving as a marker of DNA replication and cellular proliferation. Incorporated BrdU can be detected using anti-BrdU antibodies following DNA denaturation, enabling visualization or quantification of proliferating cells through immunochemical detection methods such as immunofluorescence or immunohistochemistry.
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Filamentous Fungal Mold Culture and Sporulation
Filamentous fungal mold culture and sporulation assays grow hyphae under defined nutritional and environmental conditions until asexual spores, commonly conidia, are produced; the main readouts are colony growth, sporulation onset, conidial yield, conidial morphology, viability, and, when relevant, downstream infectivity or stress phenotype.
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (605 KB)
- English - EN (605 KB)
- Français - FR (605 KB)
- Deutsch - DE (605 KB)
- Norwegian - NO (605 KB)
- Español - ES (605 KB)
- Swedish - SV (605 KB)
- Italian - IT (605 KB)
- Korean - KR (605 KB)
- Portuguese - PT (605 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.1853 mL | 40.9266 mL | 81.8532 mL | 204.6329 mL |
| 5 mM | 1.6371 mL | 8.1853 mL | 16.3706 mL | 40.9266 mL | |
| 10 mM | 0.8185 mL | 4.0927 mL | 8.1853 mL | 20.4633 mL | |
| 15 mM | 0.5457 mL | 2.7284 mL | 5.4569 mL | 13.6422 mL | |
| 20 mM | 0.4093 mL | 2.0463 mL | 4.0927 mL | 10.2316 mL | |
| 25 mM | 0.3274 mL | 1.6371 mL | 3.2741 mL | 8.1853 mL | |
| 30 mM | 0.2728 mL | 1.3642 mL | 2.7284 mL | 6.8211 mL | |
| 40 mM | 0.2046 mL | 1.0232 mL | 2.0463 mL | 5.1158 mL | |
| 50 mM | 0.1637 mL | 0.8185 mL | 1.6371 mL | 4.0927 mL | |
| 60 mM | 0.1364 mL | 0.6821 mL | 1.3642 mL | 3.4105 mL | |
| 80 mM | 0.1023 mL | 0.5116 mL | 1.0232 mL | 2.5579 mL | |
| 100 mM | 0.0819 mL | 0.4093 mL | 0.8185 mL | 2.0463 mL |
2,3,5-Trimethylpyrazine Related Classifications
HY-W010476 Related Classifications
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Keywords
- 2,3,5-Trimethylpyrazine
- 14667-55-1
- Endogenous Metabolite
- Fungal
- zearalenone
- hamster oviductal explants
- ethanol and acetaldehyde metabolism
- MES-induced seizures
- Fusarium graminearum
- OR5K1
- ADH- and ALDH-mediated oxidation
- fear-associated behaviors
- ciliary beat frequency
- chorioallantoic membrane
- Inhibitor
- inhibitor
- inhibit