2'-Deoxytubercidin 5'-triphosphate
2'-Deoxytubercidin 5'-triphosphate (C7dATP) is a deoxyadenosine triphosphate (dATP) analog that serves as a substrate for DNA polymerases. 2'-Deoxytubercidin 5'-triphosphate eliminates Hoogsteen base-pairing capacity and weakens base-stacking interactions; it is recognized and incorporated into nascent DNA strands by DNA polymerases such as Klenow and T7; it reduces DNA electrophoresis compression artifacts; it is effectively degraded by apyrase, thereby lowering the enzyme product inhibition effect caused by dATPaS. 2'-Deoxytubercidin 5'-triphosphate reduces abnormal electrophoretic mobility caused by compression of G or A residues and is used to improve the quality of DNA sequencing data.
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- CAS No.: 67460-15-5
- Formula: C11H17N4O12P3
- Molecular Weight:490.19
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
Description
In Vitro
2'-Deoxytubercidin 5'-triphosphate (C7dATP) exhibits very low substrate activity toward firefly luciferase, with a relative luminescence value of 0.006% (with ATP set as 100%); it exerts only a weak inhibitory effect on apyrase activity, as its intermediate hydrolysis product c7dADP is efficiently degraded by apyrase[1].
2'-Deoxytubercidin 5'-triphosphate is a fully functional and high-performance alternative to dATPaS, which supports long-read pyrosequencing on various microbial, viral, and plasmid DNA templates[1].
2'-Deoxytubercidin 5'-triphosphate resolves A-related electrophoretic band compression in radiolabeled chain-termination sequencing of the 4 kbp pRK plasmid insert DNA that cannot be corrected by 7-deaza-dGTP alone[2].
2'-Deoxytubercidin 5'-triphosphate eliminates band compression associated with A and improves the accuracy of automatic base calling in fluorescence sequencing[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 67460-15-5
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Molecular Weight 490.19
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Formula C11H17N4O12P3
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SMILES
O=P(O)(O)OP(OP(OC[C@H]1O[C@@H](N2C3=NC=NC(N)=C3C=C2)C[C@@H]1O)(O)=O)(O)=O
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Synonyms
C7dATP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Agarose gel electrophoresis (200 bp-5 kb)
Agarose gel electrophoresis is a molecular biology technique that uses agarose gel as a support medium to separate DNA fragments through a combination of molecular sieving and charge effects.
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Polyacrylamide gel electrophoresis (PAGE) (<1 kb)
Gel electrophoresis is a method for separating biological macromolecules (such as nucleic acids or proteins) by forcing them through a gel matrix under an electric field.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2'-Deoxytubercidin 5'-triphosphate Related Classifications
HY-131740 Related Classifications
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Keywords
- 2'-Deoxytubercidin 5'-triphosphate
- 67460-15-5
- C7dATP
- Nucleoside Antimetabolite/Analog
- DNA/RNA Synthesis
- Biochemical Assay Reagents
- apyrase
- Hoogsteen base pairing
- deoxyadenosine triphosphate
- firefly luciferase
- XbaI restriction endonuclease
- Taq polymerase
- DNA synthesis
- Klenow DNA polymerase
- T7 DNA polymerase
- PCR
- Inhibitor
- inhibitor
- inhibit