2'-O-MB-cAMP sodium
Based on 1 Customer Validation
2'-O-MB-cAMP (2'-O-Monobutyryl cyclic AMP) sodium is an inactive analog of Cyclic AMP. 2'-O-MB-cAMP sodium serves as a reference standard in FAB/MIKE mass spectrometry for the characterization of butyryl derivatives of cyclic nucleotides. 2'-O-MB-cAMP sodium does not induce differentiation or upregulate Nm23-R1 in glioma cells. 2'-O-MB-cAMP sodium can be used as a negative control cAMP analog for glioma-related research.
For research use only. We do not sell to patients.
- Purity : 99.95%
- CAS No.: 55443-13-5
- Formula: C14H17N5NaO7P
- Molecular Weight:421.28
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Chemical Information
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CAS No. 55443-13-5
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Appearance Solid
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Molecular Weight 421.28
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Formula C14H17N5NaO7P
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Color White to off-white
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SMILES
CCCC(O[C@H]1[C@@](N2C3=NC=NC(N)=C3N=C2)([H])O[C@@]4([H])[C@@]1([H])OP(OC4)(O[Na])=O)=O
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Synonyms
2'-O-Monobutyryl cyclic AMP sodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (237.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3737 mL | 11.8686 mL | 23.7372 mL | 59.3430 mL |
| 5 mM | 0.4747 mL | 2.3737 mL | 4.7474 mL | 11.8686 mL | |
| 10 mM | 0.2374 mL | 1.1869 mL | 2.3737 mL | 5.9343 mL | |
| 15 mM | 0.1582 mL | 0.7912 mL | 1.5825 mL | 3.9562 mL | |
| 20 mM | 0.1187 mL | 0.5934 mL | 1.1869 mL | 2.9671 mL | |
| 25 mM | 0.0949 mL | 0.4747 mL | 0.9495 mL | 2.3737 mL | |
| 30 mM | 0.0791 mL | 0.3956 mL | 0.7912 mL | 1.9781 mL | |
| 40 mM | 0.0593 mL | 0.2967 mL | 0.5934 mL | 1.4836 mL | |
| 50 mM | 0.0475 mL | 0.2374 mL | 0.4747 mL | 1.1869 mL | |
| 60 mM | 0.0396 mL | 0.1978 mL | 0.3956 mL | 0.9890 mL | |
| 80 mM | 0.0297 mL | 0.1484 mL | 0.2967 mL | 0.7418 mL | |
| 100 mM | 0.0237 mL | 0.1187 mL | 0.2374 mL | 0.5934 mL |
2'-O-MB-cAMP sodium Related Classifications
HY-131763 Related Classifications
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Keywords
- 2'-O-MB-cAMP
- 55443-13-5
- 2'-O-Monobutyryl cyclic AMP
- Biochemical Assay Reagents
- negative control cAMP analog
- HCN2/CRP fluorescence polarization assay
- FAB/MIKE mass spectrometry
- HCN2 cyclic nucleotide binding domain
- glioma cells
- glioma
- cyclic nucleotide analog
- butyryl derivatives of cyclic nucleotides
- Nm23-R1
- rat C6 glioma cells
- Inhibitor
- inhibitor
- inhibit