25S-Anguivioside XV
25S-Anguivioside XV (Compound 8) is a steroidal glycoside. 25S-Anguivioside XV is isolated from the unripe berries of Solanum nigrum L. 25S-Anguivioside XV inhibits LPS-induced NO production with an IC50 of 49.35 μM. 25S-Anguivioside XV exhibits antiproliferative activity against hepatoma cells.
For research use only. We do not sell to patients.
- CAS No.: 2382709-25-1
- Formula: C51H82O22
- Molecular Weight:1047.18
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
25S-Anguivioside XV (2.5-50 μM) moderately inhibits LPS-induced NO production in RAW 264.7 macrophages, with an IC50 value of 49.35 μM[1].
25S-Anguivioside XV exerts weak inhibitory effects on the proliferation of HepG2 cells, with an IC50 value of 43.74 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2382709-25-1
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Molecular Weight 1047.18
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Formula C51H82O22
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SMILES
C[C@@]12[C@](CC([C@]2([H])[C@H](C)C(CC[C@H](C)CO[C@@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)=O)=O)([H])[C@@]4([H])[C@@](CC1)([H])[C@@]5(C(C[C@H](CC5)O[C@@H]6O[C@@H]([C@H]([C@@H]([C@H]6O[C@@H]7O[C@H]([C@@H]([C@H]([C@H]7O)O)O)C)O)O[C@@H]8O[C@H]([C@@H]([C@H]([C@H]8O)O)O)C)CO)=CC4)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
25S-Anguivioside XV Related Classifications
HY-N19427 Related Classifications
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