Art. -Nr.: HY-18569

3-Indoleacetic acid

Name: 3-Indoleacetic acid
Brand: MedChemExpress
SKU: HY-18569
Price: 50.00 USD
Availability: InStock
Rating: 5 (25 reviews)

(Synonyms: Indole-3-acetic acid; 3-IAA)

25 Cited Publications

Kundenbewertung

Based on 25 publication(s) in Google Scholar

3-Indoleacetic acid is is an IAA hormone and growth regulator that can promote plant nutritional growth through processes such as cell expansion, differentiation, morphogenesis, and organogenesis.

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Reinheit : 99.94%
CAS. Nr.: 87-51-4
Formel: C₁₀H₉NO₂
Molecular Weight:175.19

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

3-Indoleacetic acid Chemical Structure

CAS. Nr.: 87-51-4

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (25)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll (1)
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

3-Indoleacetic acid is is an IAA hormone and growth regulator that can promote plant nutritional growth through processes such as cell expansion, differentiation, morphogenesis, and organogenesis^[2].

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

3-Indoleacetic acid promotes the enrichment of salt tolerant bacteria with growth promoting function during the secondary fermentation process of compost^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

In Vivo

3-Indoleacetic acid (50 g/ha) improves outcrossing rate of diverse rice cytoplasmic male sterile lines and hybrid rice seed production through affecting floral traits^[2].
3-Indoleacetic acid (10 mg/kg) improves photosynthetic performance and reduces the toxic effect of cadmium on C. camphora^[3].
3-Indoleacetic acid (500 mg/kg, once daily, 14 days, p.o.) exhibits adverse effects on liver and kidney function, myocardial and skeletal muscles, and testes in rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

adult male Wistar albino rat^[4].
Dosage:

500 mg/kg body weight, once daily, 14 days
Administration:

oral gavage (p.o.)
Result:

Reduced the number of red blood cells and white blood cells^[4].

Chemical Information

CAS. Nr. 87-51-4
Appearance Solid
Molecular Weight 175.19
Formel C₁₀H₉NO₂
Color Off-white to light brown
SMILES

OC(CC1=CNC2=C1C=CC=C2)=O
Synonyms

Indole-3-acetic acid; 3-IAA
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (25)

All In Vivo Efficacy StudyIn Vivo ImagingCell Proliferation/Viability AssayCell Imaging/Staining

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Journal Impact Factor

Cancer Discov

Gut microbiota modulation through Akkermansia spp. supplementation increases CAR-T cell potency. [Abstract]

2025 Jun 11. PMID: 40498998
Autophagy

METTL3-dependent m6A modification of SNAP29 induces "autophagy-mitochondrial crisis" in the ischemic microenvironment after soft tissue transplantation. [Abstract]

2025 Oct;21(10):2168-2191. PMID: 40340690
Exp Mol Med

Proline/serine-rich coiled-coil 1 alleviates atherosclerosis via remodeling tryptophan metabolism mediated by Akkermansia muciniphila. [Abstract]

2026 Mar;58(3):848-863. PMID: 41792233
Adv Sci (Weinh)

Colorectal Cancer Cell's Weapon: RNF32 Engages SPP1+ Macrophages to Foster Liver Metastasis, Targeted by Indole-3-Acetic Acid. [Abstract]

2025 Dec 12:e19735. PMID: 41387204
Adv Sci (Weinh)

Gut Parabacteroides distasonis-derived Indole-3-Acetic Acid Promotes Phospholipid Remodeling and Enhances Ferroptosis Sensitivity via the AhR-FASN Axis in Bladder Cancer. [Abstract]

2025 Sep;12(34):e04688. PMID: 40557796

3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep;12(34):e04688. [Abstract]

3-IAA (50, 100 mg/kg; once daily; 18 days) significantly inhibited tumor growth in a dose-dependent manner.

3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep;12(34):e04688. [Abstract]

3-IAA (50, 100 mg/kg; once daily; 6 weeks) significantly reduced bioluminescent signal in the footpads and popliteal lymph nodes; the higher the concentration, the more significant the reduction.

3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep;12(34):e04688. [Abstract]

The proliferation of T24 and 5637 cells after treatment with different concentrations of 3-IAA for 24 or 48 hours was evaluated using the CCK-8 assay.

3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep;12(34):e04688. [Abstract]

Representative images of T24 and 5637 cells were observed after treatment with different concentrations of 3-IAA (300 µM and 600 µM; 24 h) or DMSO.

3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep;12(34):e04688. [Abstract]

Representative images of wound healing assays were observed after treating T24 and 5637 cells with different concentrations of 3-IAA (300 µM and 600 µM; 24 h) or DMSO.
Plant Commun

Brassinosteroid signaling regulates PIN5/10-mediated intracellular auxin homeostasis to promote lateral root development in tomato. [Abstract]

2026 Mar 16:101823. PMID: 41846312
Mater Today Bio

Inhaled PAMAM-based nano-formulation prolonged lung retention for alleviating pulmonary inflammation of COPD. [Abstract]

2025 May 6:32:101845. PMID: 40469693
Clin Cancer Res

Dual Inhibition of TRIP13 and Aurora A Induces Mitotic DNA Damage and Concurrent Pyroptotic-apoptotic Cell Death in Rb-deficient Cancer Cells. [Abstract]

2026 Apr 21:10.1158/1078-0432.CCR-25-4712. PMID: 42013305
Proc Natl Acad Sci U S A

The RPA-RNF20-SNF2H cascade promotes proper chromosome segregation and homologous recombination repair. [Abstract]

2023 May 16;120(20):e2303479120. PMID: 37155876
Diabetes

Gut microbiota-tryptophan metabolism-GLP-1 axis participates in β-cell regeneration induced by dapagliflozin. [Abstract]

2024 Jun 1;73(6):926-940. PMID: 38471012
Food Res Int

2026 Feb 17.
Free Radic Biol Med

Gut microbial metabolite alleviates osteoporosis by attenuating AKT-NFATc1 signaling pathway and ROS production. [Abstract]

2025 Nov 23:243:351-366. PMID: 41290102
Cell Rep

Aryl hydrocarbon receptor-induced activation of AIM2 inflammasome is mediated by MOMP and MPT: A vital therapeutic pathway for inflammation. [Abstract]

2025 Dec 10;44(12):116673. PMID: 41385364
Cell Rep

Bifidobacterium animalis suppresses non-small cell lung cancer progression and modulates tumor immunity through indole-3-acetic acid. [Abstract]

2025 Aug 12;44(8):116132. PMID: 40802513
Cell Prolif

Tryptophan Metabolic Enzyme IL4I1 Inhibits Ferroptosis by Decreasing Ubiquitination of Nrf2 via I3P in Glioblastoma. [Abstract]

2025 Jun;58(6):e13816. PMID: 40071723
Food Sci Hum Wellness

2026 Apr 3.
J Agric Food Chem

2026 Jul 13. PMID: 42439058
Animal Research and One Health

2026 Jun 2.
Mol Med Rep

Saikosaponin A protects against uremic toxin indole‑3 acetic acid‑induced damage to the myocardium. [Abstract]

2023 Sep;28(3):159. PMID: 37417356
Sci Rep

Multi-omics study reveals gut microbiota dysbiosis and tryptophan metabolism alterations in GH-PitNET progression. [Abstract]

2025 Jul 7;15(1):24261. PMID: 40624207
Plants

Integrated Multi-Omics Analysis Revealed the Synergistic Regulatory Mechanisms of Salt Tolerance in Soybean (Kefeng 1). [Abstract]

2026 Feb 10;15(4):555. PMID: 41754262
Urolithiasis

Gut microbiota-derived indole-3-acetic acid ameliorates calcium oxalate renal stone formation via AHR/NF‑κB axis. [Abstract]

2025 Jul 2;53(1):134. PMID: 40601009
bioRxiv

2026 Mar 20.
ChemRxiv

2025 Apr 11.
Research Square Preprint

2021 Aug.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (713.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (5.71 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.7081 mL

28.5404 mL

57.0809 mL

5 mM

1.1416 mL

5.7081 mL

11.4162 mL

10 mM

0.5708 mL

2.8540 mL

5.7081 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (11.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (11.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (11.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Corn Oil

Solubility: 6.67 mg/mL (38.07 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 50 mg/mL (285.40 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

All Signal Pathway Related

Research Protocol for Endocrine Diseases
Endocrine diseases often arise from disrupted hormone production, hormone signaling, or target-tissue responsiveness; for diabetes-focused endocrine disease models, insulin signaling regulates glucose uptake, hepatic glucose output, lipid metabolism, and β-cell compensation. Type 2 diabetes develops through interacting defects in insulin resistance, β-cell dysfunction, adipose inflammation, hepatic glucose overproduction, altered incretin signaling, and ectopic lipid metabolism. A major unresolved question is whether endocrine dysfunction is driven primarily by target-tissue insulin resistance, intrinsic β-cell failure, immune/inflammatory stress, or combined multi-organ failure that differs by disease stage.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Li Y, et al. Inoculating indoleacetic acid bacteria promotes the enrichment of halotolerant bacteria during secondary fermentation of composting. J Environ Manage. 2022 Nov 15;322:116021. [Content Brief]

[2]. ElShamey EAZ, et al. Growth Regulators Improve Outcrossing Rate of Diverse Rice Cytoplasmic Male Sterile Lines through Affecting Floral Traits. Plants (Basel). 2022 May 12;11(10):1291. [Content Brief]

[3]. Zhou J, et al. Effects of exogenous 3-indoleacetic acid and cadmium stress on the physiological and biochemical characteristics of Cinnamomum camphora. Ecotoxicol Environ Saf. 2020 Mar 15;191:109998. [Content Brief]

[4]. Ismail HTH. Assessment toxic effects of exposure to 3-indoleacetic acid via hemato-biochemical, hormonal, and histopathological screening in rats. Environ Sci Pollut Res Int. 2022 Dec;29(60):90703-90718. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	5.7081 mL	28.5404 mL	57.0809 mL	142.7022 mL
H₂O / DMSO	5 mM	1.1416 mL	5.7081 mL	11.4162 mL	28.5404 mL
DMSO	10 mM	0.5708 mL	2.8540 mL	5.7081 mL	14.2702 mL
	15 mM	0.3805 mL	1.9027 mL	3.8054 mL	9.5135 mL
	20 mM	0.2854 mL	1.4270 mL	2.8540 mL	7.1351 mL
	25 mM	0.2283 mL	1.1416 mL	2.2832 mL	5.7081 mL
	30 mM	0.1903 mL	0.9513 mL	1.9027 mL	4.7567 mL
	40 mM	0.1427 mL	0.7135 mL	1.4270 mL	3.5676 mL
	50 mM	0.1142 mL	0.5708 mL	1.1416 mL	2.8540 mL
	60 mM	0.0951 mL	0.4757 mL	0.9513 mL	2.3784 mL
	80 mM	0.0714 mL	0.3568 mL	0.7135 mL	1.7838 mL
	100 mM	0.0571 mL	0.2854 mL	0.5708 mL	1.4270 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

Keywords

3-Indoleacetic acid

25 Cited Publications

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3-Indoleacetic acid

Publications Citing Use of MedChemExpress (MCE) 3-Indoleacetic acid

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen