3-Chloro-5-hydroxybenzoic acid
Based on 4 publication(s) in Google Scholar
3-Chloro-5-hydroxybenzoic acid is a potent, orally active and selective lactate receptor GPR81 agonist, with an EC50 of 16 μM for human GPR81. 3-Chloro-5-hydroxybenzoic acid exhibits favorable in vivo effects on lipolysis in a mouse model of obesity.
For research use only. We do not sell to patients.
- Purity: 99.54%
- CAS No.: 53984-36-4
- Formula: C7H5ClO3
- Molecular Weight:172.57
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 3-Chloro-5-hydroxybenzoic acid
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Biological Activity
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GPR81 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COS-7 | EC50 |
16 μM
Compound: 2
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Agonist activity at human recombinant GPR81 transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay
Agonist activity at human recombinant GPR81 transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay
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[PMID: 24900524] |
| COS-7 | EC50 |
>1000 μM
Compound: 2
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Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay
Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay
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[PMID: 24900524] |
3-Chloro-5-hydroxybenzoic acid shows potency against human (EC50=16 μM), monkey (EC50=17 μM), dog (EC50=67 μM), rat (EC50=7 μM), mouse (EC50=22 μM), and hamster GPR81 (EC50=27 μM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pharmacokinetic Parameters of 3-Chloro-5-hydroxybenzoic acid in Mice[1].
| dose (mg/kg) | tmax (h) | AUCinf (h ng/mL) | Cmax (ng/mL) | Cmax (μM) | t1/2 (h) |
| 30 | 0.5 | 9356 | 11689.50 | 67.2 | 1.47 |
| 100 | 0.5 | 51312 | 55252.80 | 341.9 | 1.10 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57Bl6/J mice (DIO mice)[1]
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Dosage:30, 100, and 300 mg/kg
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Administration:PO in overnight fasted C57Bl6/J mice fed high fat chow for 10 weeks
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Result:Significant reductions in nonesterified free fatty acid cholesterol (NEFAc) at all doses tested.
Chemical Information
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CAS No. 53984-36-4
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Appearance Solid
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Molecular Weight 172.57
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Formula C7H5ClO3
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Color White to light brown
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SMILES
ClC1=CC(O)=CC(C(O)=O)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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PLoS Biol
Structures of G-protein coupled receptor HCAR1 in complex with Gi1 protein reveal the mechanistic basis for ligand recognition and agonist selectivity. [Abstract]2025 Apr 15;23(4):e3003126. PMID: 40233099 -
Inflamm Res
Lactate acts as a metabolic brake on inflammation by repressing NLRP3 transcription via NF-κB inhibition. [Abstract]2026 Jun 11;75(1):137. PMID: 42274732 -
J Pathol
Lactate-mediated activation of GPR81 regulates BCR/Abl protein expression in chronic myeloid leukemia cells selected under low oxygen tension. [Abstract]2025 Oct 23. PMID: 41128634 -
Food Sci Nutr
Octanoic Acid-Rich Enteral Nutrition Alleviates Sepsis-Associated Metabolic Disorders by Suppressing White Adipose Tissue Browning in Mice. [Abstract]2026 Jun 9;14(6):e72020. PMID: 42282442
Solvent & Solubility
DMSO : 100 mg/mL (579.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (615 KB)
- English - EN (615 KB)
- Français - FR (615 KB)
- Deutsch - DE (615 KB)
- Norwegian - NO (615 KB)
- Español - ES (615 KB)
- Swedish - SV (615 KB)
- Italian - IT (615 KB)
- Korean - KR (615 KB)
- Portuguese - PT (615 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.7947 mL | 28.9737 mL | 57.9475 mL | 144.8687 mL |
| 5 mM | 1.1589 mL | 5.7947 mL | 11.5895 mL | 28.9737 mL | |
| 10 mM | 0.5795 mL | 2.8974 mL | 5.7947 mL | 14.4869 mL | |
| 15 mM | 0.3863 mL | 1.9316 mL | 3.8632 mL | 9.6579 mL | |
| 20 mM | 0.2897 mL | 1.4487 mL | 2.8974 mL | 7.2434 mL | |
| 25 mM | 0.2318 mL | 1.1589 mL | 2.3179 mL | 5.7947 mL | |
| 30 mM | 0.1932 mL | 0.9658 mL | 1.9316 mL | 4.8290 mL | |
| 40 mM | 0.1449 mL | 0.7243 mL | 1.4487 mL | 3.6217 mL | |
| 50 mM | 0.1159 mL | 0.5795 mL | 1.1589 mL | 2.8974 mL | |
| 60 mM | 0.0966 mL | 0.4829 mL | 0.9658 mL | 2.4145 mL | |
| 80 mM | 0.0724 mL | 0.3622 mL | 0.7243 mL | 1.8109 mL | |
| 100 mM | 0.0579 mL | 0.2897 mL | 0.5795 mL | 1.4487 mL |
3-Chloro-5-hydroxybenzoic acid Related Classifications
HY-W016868 Related Classifications
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