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Cat. No.: HY-W837564

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4-Carene

4-Carene is an anticancer and Antifungal agent. 4-Carene can be isolated from the essential oils of Litsea cubeba and Chenopodium ambrosioides. 4-Carene can be used in research on lung cancer.

For research use only. We do not sell to patients.

CAS No.: 29050-33-7
Formula: C₁₀H₁₆
Molecular Weight:136.23

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

4-Carene Chemical Structure

CAS No.: 29050-33-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	LC₅₀	250.17 μg/mL	Cytotoxicity against human A549 lung carcinomatous alveolar basal epithelial cells assessed as cell viability reduction incubated 24 hrs by MTT assay. Cytotoxicity against human A549 lung carcinomatous alveolar basal epithelial cells assessed as cell viability reduction incubated 24 hrs by MTT assay.	28356026

In Vitro

Anti-cancer

4-Carene (5-250 µg/mL; 24 h) reduces A549 cell viability in a dose-dependent manner with an LC₅₀ of 250.17 µg/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

A549 cell line
Concentration:

5, 10, 20, 50, 100, 200, and 250 µg/mL
Incubation Time:

24 h
Result:

The LC₅₀ against A549 cells was 250.17 µg/mL.
Suppressed cell proliferation/viability and induced cell death in a dose-dependent manner, with lower cell death than 3-methylcyclohexanone (80) and thujopsene (37).

Chemical Information

CAS No. 29050-33-7
Molecular Weight 136.23
Formula C₁₀H₁₆
SMILES

CC1CC(C2(C)C)C2C=C1
Structure Classification
- Terpenoids
- Other Monoterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bordoloi M, et al. Volatile Inhibitors of Phosphatidylinositol-3-Kinase (PI3K) Pathway: Anticancer Potential of Aroma Compounds of Plant Essential Oils. Anti-cancer agents in medicinal chemistry. 2018;18(1):87-109.

[2]. Ali N, et al. GC/MS analysis, anti-leishmanial and relaxant activity of essential oil of Chenopodium ambrosioides (L.) from Malakand region. Pakistan journal of pharmaceutical sciences. 2021 Mar;34(2):577-583.

[3]. Prabhu V, et al. Chemical composition and dual bioefficacy of Chenopodium ambrosioides essential oil: Anti-inflammatory and cytotoxic potential. Plant Science Today. 2025;12.

[4]. Prabhu V, et al. Chemical composition and dual bioefficacy of Chenopodium ambrosioides essential oil: Anti-inflammatory and cytotoxic potential[J]. Plant Science Today, 2025, 12.

[5]. Boutkhil S, et al. Chemical composition and antimicrobial activity of crude, aqueous, ethanol extracts and essential oils of Dysphania ambrosioides (L.) Mosyakin & Clemants[J]. Acta botanica gallica, 2009, 156(2): 201-209.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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