2. GPCR/G Protein
  3. Free Fatty Acid Receptor
  4. 4-CMTB

4-CMTB is a selective free fatty acid receptor 2 (FFA2/GPR43) agonist and a positive allosteric modulator (pEC50=6.38).

For research use only. We do not sell to patients.

CAS No. : 300851-67-6

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Customer Review

Based on 6 publication(s) in Google Scholar

4-CMTB Related Antibodies

Top Publications Citing Use of Products

    4-CMTB purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157523.  [Abstract]

    THP (Tetrastigma hemsleyanum polysaccharides, THP) or 4-CMTB (10 mg/kg, i.p., 5 days) improved the Disease Activity Index induced by DSS. However, GLPG0974 (1 mg/kg, i.g., 5 days) reversed the Disease Activity Index increase induced by Tetrastigma hemsleyanum polysaccharides in the IBD mouse model.

    4-CMTB purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157523.  [Abstract]

    THP(Tetrastigma hemsleyanum polysaccharides, THP) or 4-CMTB (10 mg/kg, i.p., 5 days) reduced the expression of these pro-inflammatory mediators (IL-6, IL-1β, TNFα, and MCP-1) in the colon. Conversely, mice administered THP + GLPG0974 (1 mg/kg, i.g., 5 days) displayed an upregulation of colonic cytokine mRNA.

    4-CMTB purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157523.  [Abstract]

    THP (Tetrastigma hemsleyanum polysaccharides, THP)or 4-CMTB (10 mg/kg, i.p., 5 days) reduced the abnormal spleen index induced by DSS and improved the pathological changes in the spleens of mice following DSS treatment. Conversely, in the THP + GLPG0974 group, the abnormal spleen index and spleen inflammation persisted, indicating that GLPG0974 reversed the therapeutic effect of THP.

    4-CMTB purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157523.  [Abstract]

    Immunohistochemical staining images of Ki67 showed that THP (Tetrastigma hemsleyanum polysaccharides, THP) or 4-CMTB (10 mg/kg, i.p., 5 days) regulated the growth and apoptosis of intestinal epithelial cells induced by DSS in mice. However, mice in the THP + GLPG0974 group exhibited persistent damage to intestinal epithelial cells.

    4-CMTB purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157523.  [Abstract]

    THP (Tetrastigma hemsleyanum polysaccharides, THP) or 4-CMTB alleviated the aberrant proliferation of CD3+ cells induced by DSS in mice. However, mice in the THP + GLPG0974 group still exhibited elevated levels of CD3+ cells.

    View All Free Fatty Acid Receptor Isoform Specific Products:

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    FFAR1 FFAR2 FFAR3 FFAR4

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    4-CMTB is a selective free fatty acid receptor 2 (FFA2/GPR43) agonist and a positive allosteric modulator (pEC50=6.38)[1].

    Cellular Effect
    Cell Line Type Value Description References
    CHO IC50
    0.8 μM
    Compound: rac-1
    Agonist activity at human FFA2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
    Agonist activity at human FFA2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
    		[PMID: 20005104]
    In Vitro

    4-CMTB is both a direct agonist and a positive allosteric modulator of the action of short-chain free fatty acids. 4-CMTB modulates the activity of short-chain fatty acids at FFA2 via the FFA2 second extracellular loop (ECL2)[1][2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    4-CMTB Related Antibodies
    Molecular Weight

    294.80

    Formula

    C14H15ClN2OS
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NC1=NC=CS1)C(C(C)C)C2=CC=C(Cl)C=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (339.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3921 mL 16.9607 mL 33.9213 mL
    5 mM 0.6784 mL 3.3921 mL 6.7843 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (8.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.66%

    SDS (393 KB)

    COA (254 KB) HNMR (254 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.3921 mL 16.9607 mL 33.9213 mL 84.8033 mL
    5 mM 0.6784 mL 3.3921 mL 6.7843 mL 16.9607 mL
    10 mM 0.3392 mL 1.6961 mL 3.3921 mL 8.4803 mL
    15 mM 0.2261 mL 1.1307 mL 2.2614 mL 5.6536 mL
    20 mM 0.1696 mL 0.8480 mL 1.6961 mL 4.2402 mL
    25 mM 0.1357 mL 0.6784 mL 1.3569 mL 3.3921 mL
    30 mM 0.1131 mL 0.5654 mL 1.1307 mL 2.8268 mL
    40 mM 0.0848 mL 0.4240 mL 0.8480 mL 2.1201 mL
    50 mM 0.0678 mL 0.3392 mL 0.6784 mL 1.6961 mL
    60 mM 0.0565 mL 0.2827 mL 0.5654 mL 1.4134 mL
    80 mM 0.0424 mL 0.2120 mL 0.4240 mL 1.0600 mL
    100 mM 0.0339 mL 0.1696 mL 0.3392 mL 0.8480 mL
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    4-CMTB Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    4-CMTB300851-67-6Free Fatty Acid ReceptorFFARligandGPCRsECL2short-chainfreefattyacidsago-allostericInhibitorinhibitorinhibit

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