5-Iodouracil
Based on 1 Customer Validation
5-Iodouracil is an iodinated uracil pyrimidine base and a thymine analog. 5-Iodouracil exerts its lethal effect by substituting for thymine and being incorporated into phage DNA. 5-Iodouracil does not interfere with the host cell's transfer of thymine to progeny phages, nor does it alter lysis time or phage DNA content. 5-Iodouracil has no direct effect on phage particles, adsorption, or plating efficiency. Short oligonucleotide prodrugs containing 5-Fluoro and 5-Iodouracil are able to exert anticancer effects in a photoresponsive manner.
For research use only. We do not sell to patients.
- Purity : 99.95%
- CAS No.: 696-07-1
- Formula: C4H3IN2O2
- Molecular Weight:237.98
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-I0626
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Biological Activity
Description
In Vitro
5-Iodouracil (2-10 μM) supports particle production on a molar basis equivalently to thymine in T4td8-infected E. coli BT-, but reduces viable progeny[3].
5-Iodouracil (30-1000 μM) does not impair the transfer of [3H] thymine to progeny phages in T4td8-infected E. coli BT- host cells[3].
5-Iodouracil (100 μM) does not alter net phage DNA synthesis in T4td8-infected E. coli BT-[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 696-07-1
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Appearance Solid
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Molecular Weight 237.98
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Formula C4H3IN2O2
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Color White to off-white
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SMILES
O=C1NC=C(I)C(=O)N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (105.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.51 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.51 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. Calabresi P, et al. Initial clinical studies with 5-iodo-2′-deoxyuridine. Cancer research. 1961 May 1;21(4):550-9. [Content Brief]
[3]. Byrd DM, et al. The effect of 5-iodouracil on the growth and biosynthetic processes of bacteriophage T4td8 in the absence of light. Chemico-Biological Interactions. 1976 Feb 1;12(2):197-210. [Content Brief]
[4]. Krenitsky TA, et al. 3'-Amino-2',3'-dideoxyribonucleosides of some pyrimidines: synthesis and biological activities. Journal of medicinal chemistry. 1983 Jun;26(6):891-5. [Content Brief]
[5]. Fujimoto K, et al. Short oligonucleotide prodrug having 5-fluoro and 5-iodouracil inhibits the proliferation of cancer cells in a photo-responsive manner. Bioorganic & medicinal chemistry letters. 2014 Aug 15;24(16):3736-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.2020 mL | 21.0102 mL | 42.0203 mL | 105.0508 mL |
| 5 mM | 0.8404 mL | 4.2020 mL | 8.4041 mL | 21.0102 mL | |
| 10 mM | 0.4202 mL | 2.1010 mL | 4.2020 mL | 10.5051 mL | |
| 15 mM | 0.2801 mL | 1.4007 mL | 2.8014 mL | 7.0034 mL | |
| 20 mM | 0.2101 mL | 1.0505 mL | 2.1010 mL | 5.2525 mL | |
| 25 mM | 0.1681 mL | 0.8404 mL | 1.6808 mL | 4.2020 mL | |
| 30 mM | 0.1401 mL | 0.7003 mL | 1.4007 mL | 3.5017 mL | |
| 40 mM | 0.1051 mL | 0.5253 mL | 1.0505 mL | 2.6263 mL | |
| 50 mM | 0.0840 mL | 0.4202 mL | 0.8404 mL | 2.1010 mL | |
| 60 mM | 0.0700 mL | 0.3502 mL | 0.7003 mL | 1.7508 mL | |
| 80 mM | 0.0525 mL | 0.2626 mL | 0.5253 mL | 1.3131 mL | |
| 100 mM | 0.0420 mL | 0.2101 mL | 0.4202 mL | 1.0505 mL |
5-Iodouracil Related Classifications
HY-W001983 Related Classifications
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