Cat. No.: HY-W267446

6-Methoxy-4-methylcoumarin

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6-Methoxy-4-methylcoumarin is a 4-methylcoumarin derivative with an antitumor activity. 6-Methoxy-4-methylcoumarin activates melanin synthesis in B16F10 melanoma cells. 6-Methoxy-4-methylcoumarin is a pigmentation stimulator, and can be used for the study of skin conditions such as vitiligo.

For research use only. We do not sell to patients.

Purity: 99.00%
CAS No.: 6295-35-8
Formula: C₁₁H₁₀O₃
Molecular Weight:190.20

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

6-Methoxy-4-methylcoumarin Chemical Structure

CAS No.: 6295-35-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Melanocortin Receptor Isoforms :

All GSK-3 Isoforms :

In Vitro

6-Methoxy-4-methylcoumarin (25-400 μM; 72 hours) demonstrates a survival rate of 67.95% at 200 μM and 90.38% at 100 μM. 6-Methoxy-4-methylcoumarin significantly stimulates melanin production in a concentration-dependent manner at 25, 50, and 100 μM. 6-Methoxy-4-methylcoumarin enhances tyrosinase activity in B16F10 cells, subsequently stimulating melanin production^[1].
6-Methoxy-4-methylcoumarin (25-100 μM; 4 hours) inhibits ERK and AKT phosphorylation, which leads to the upregulation of MITF and TRP proteins. 6-Methoxy-4-methylcoumarin activates GSK3β phosphorylation, reduces β-catenin phosphorylation, and stimulates melanogenesis via the GSK3β/β-catenin pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

B16F10 melanoma cells
Concentration:

25 μM, 50 μM, 100 μM, 200 μM, 400 μM
Incubation Time:

72 hours
Result:

Demonstrated a survival rate of 67.95% at 200 μM and 90.38% at 100 μM.

Western Blot Analysis^[1]

Cell Line:

B16F10 melanoma cells
Concentration:

25 μM, 50 μM, 100 μM
Incubation Time:

4 hours
Result:

Decreased AKT and ERK phosphorylation.

Chemical Information

CAS No. 6295-35-8
Appearance Solid
Molecular Weight 190.20
Formula C₁₁H₁₀O₃
Color Off-white to light yellow
SMILES

CC1=CC(=O)OC2=C1C=C(C=C2)OC
Structure Classification
- Phenylpropanoids
- Coumarins
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (105.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.2576 mL

26.2881 mL

52.5762 mL

5 mM

1.0515 mL

5.2576 mL

10.5152 mL

10 mM

0.5258 mL

2.6288 mL

5.2576 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lee YJ, et al. Mechanistic Insights into the Stimulatory Effect of Melanogenesis of 4-Methylcoumarin Derivatives in B16F10 Melanoma Cells. Int J Mol Sci. 2024 Nov 19;25(22):12421. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.2576 mL	26.2881 mL	52.5762 mL	131.4406 mL
	5 mM	1.0515 mL	5.2576 mL	10.5152 mL	26.2881 mL
	10 mM	0.5258 mL	2.6288 mL	5.2576 mL	13.1441 mL
	15 mM	0.3505 mL	1.7525 mL	3.5051 mL	8.7627 mL
	20 mM	0.2629 mL	1.3144 mL	2.6288 mL	6.5720 mL
	25 mM	0.2103 mL	1.0515 mL	2.1030 mL	5.2576 mL
	30 mM	0.1753 mL	0.8763 mL	1.7525 mL	4.3814 mL
	40 mM	0.1314 mL	0.6572 mL	1.3144 mL	3.2860 mL
	50 mM	0.1052 mL	0.5258 mL	1.0515 mL	2.6288 mL
	60 mM	0.0876 mL	0.4381 mL	0.8763 mL	2.1907 mL
	80 mM	0.0657 mL	0.3286 mL	0.6572 mL	1.6430 mL
	100 mM	0.0526 mL	0.2629 mL	0.5258 mL	1.3144 mL

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6-Methoxy-4-methylcoumarin

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Multi-Target Compound Library

All Melanocortin Receptor Isoforms

All GSK-3 Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

6-Methoxy-4-methylcoumarin Related Classifications

HY-W267446 Related Classifications