6-Methoxy-4-methylcoumarin
Based on 1 Customer Validation
6-Methoxy-4-methylcoumarin is a 4-methylcoumarin derivative with an antitumor activity. 6-Methoxy-4-methylcoumarin activates melanin synthesis in B16F10 melanoma cells. 6-Methoxy-4-methylcoumarin is a pigmentation stimulator, and can be used for the study of skin conditions such as vitiligo.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.00%
- CAS. Nr.: 6295-35-8
- Formel: C11H10O3
- Molecular Weight:190.20
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Speicherung:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
In Vitro
6-Methoxy-4-methylcoumarin (25-400 μM; 72 hours) demonstrates a survival rate of 67.95% at 200 μM and 90.38% at 100 μM. 6-Methoxy-4-methylcoumarin significantly stimulates melanin production in a concentration-dependent manner at 25, 50, and 100 μM. 6-Methoxy-4-methylcoumarin enhances tyrosinase activity in B16F10 cells, subsequently stimulating melanin production[1].
6-Methoxy-4-methylcoumarin (25-100 μM; 4 hours) inhibits ERK and AKT phosphorylation, which leads to the upregulation of MITF and TRP proteins. 6-Methoxy-4-methylcoumarin activates GSK3β phosphorylation, reduces β-catenin phosphorylation, and stimulates melanogenesis via the GSK3β/β-catenin pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:B16F10 melanoma cells
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Concentration:25 μM, 50 μM, 100 μM, 200 μM, 400 μM
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Incubation Time:72 hours
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Result:Demonstrated a survival rate of 67.95% at 200 μM and 90.38% at 100 μM.
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Cell Line:B16F10 melanoma cells
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Concentration:25 μM, 50 μM, 100 μM
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Incubation Time:4 hours
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Result:Decreased AKT and ERK phosphorylation.
Chemical Information
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CAS. Nr. 6295-35-8
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Appearance Solid
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Molecular Weight 190.20
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Formel C11H10O3
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Color Off-white to light yellow
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SMILES
CC1=CC(=O)OC2=C1C=C(C=C2)OC
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 20 mg/mL (105.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Subcutaneous Cell-Line-Derived Xenograft
Subcutaneous cell-line-derived xenograft (CDX) models are established by implanting cultured human cancer cell lines into immunodeficient mice, where the injected cells form localized tumors that can be monitored in vivo as a measure of tumorigenic potential, growth kinetics, and treatment response. These models are widely used in oncology research because they allow reproducible tumor formation and enable comparative assessment of tumor growth between different cell lines or genetic manipulations in a controlled in vivo microenvironment. Subcutaneous implantation of cancer cells in immunodeficient mice is a standard approach for evaluating tumor growth behavior and therapeutic response across multiple cancer types, including prostate, esophageal, pancreatic, and colon cancer models.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.2576 mL | 26.2881 mL | 52.5762 mL | 131.4406 mL |
| 5 mM | 1.0515 mL | 5.2576 mL | 10.5152 mL | 26.2881 mL | |
| 10 mM | 0.5258 mL | 2.6288 mL | 5.2576 mL | 13.1441 mL | |
| 15 mM | 0.3505 mL | 1.7525 mL | 3.5051 mL | 8.7627 mL | |
| 20 mM | 0.2629 mL | 1.3144 mL | 2.6288 mL | 6.5720 mL | |
| 25 mM | 0.2103 mL | 1.0515 mL | 2.1030 mL | 5.2576 mL | |
| 30 mM | 0.1753 mL | 0.8763 mL | 1.7525 mL | 4.3814 mL | |
| 40 mM | 0.1314 mL | 0.6572 mL | 1.3144 mL | 3.2860 mL | |
| 50 mM | 0.1052 mL | 0.5258 mL | 1.0515 mL | 2.6288 mL | |
| 60 mM | 0.0876 mL | 0.4381 mL | 0.8763 mL | 2.1907 mL | |
| 80 mM | 0.0657 mL | 0.3286 mL | 0.6572 mL | 1.6430 mL | |
| 100 mM | 0.0526 mL | 0.2629 mL | 0.5258 mL | 1.3144 mL |
6-Methoxy-4-methylcoumarin Related Classifications
HY-W267446 Related Classifications
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