Brofaromine
Based on 1 Customer Validation
Brofaromine (CGP 11305A) is a monoamine oxidase (MAO) inhibitor with IC50 of 0.2 μM for MAO-A.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.37%
- No. CAS: 63638-91-5
- Fòrmula: C14H16BrNO2
- Peso molecular:310.19
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
IC50: 0.2 μM (MAO-A)[1]
In Vitro
In order to assess whether the protective effect is related to the inhibition of the MAO-A or due to another mechanism, the effect of Brofaromine (CGP 11305A, BRO) is determined on the activity of MAO-A in cultured cortical cells. It shows, firstly, that cultured cortical cells have a significant MAO-A activity, and secondly, that Brofaromine inhibits the enzyme in a concentration-dependent manner. The IC50 is 0.19 μM for Brofaromine . Hill coefficient is 0.96. The enzyme is almost completely inhibited at 10 μM . Brofaromine inhibits MAO-A activity of our cultured cortical cells in a concentration-dependent manner. The IC50 range of Brofaromine is 0.01 μM to 0.1 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 63638-91-5
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Appearance Solid
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Peso molecular 310.19
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Fòrmula C14H16BrNO2
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Color Off-white to light yellow
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SMILES
BrC1=CC(OC)=CC2=C1OC(C3CCNCC3)=C2
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Synonyms
CGP 11305A
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
In Vitro:
DMSO : 250 mg/mL (805.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (20.15 mM); Clear solution
This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 6.25 mg/mL (20.15 mM); Clear solution
This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.2238 mL | 16.1192 mL | 32.2383 mL | 80.5958 mL |
| 5 mM | 0.6448 mL | 3.2238 mL | 6.4477 mL | 16.1192 mL | |
| 10 mM | 0.3224 mL | 1.6119 mL | 3.2238 mL | 8.0596 mL | |
| 15 mM | 0.2149 mL | 1.0746 mL | 2.1492 mL | 5.3731 mL | |
| 20 mM | 0.1612 mL | 0.8060 mL | 1.6119 mL | 4.0298 mL | |
| 25 mM | 0.1290 mL | 0.6448 mL | 1.2895 mL | 3.2238 mL | |
| 30 mM | 0.1075 mL | 0.5373 mL | 1.0746 mL | 2.6865 mL | |
| 40 mM | 0.0806 mL | 0.4030 mL | 0.8060 mL | 2.0149 mL | |
| 50 mM | 0.0645 mL | 0.3224 mL | 0.6448 mL | 1.6119 mL | |
| 60 mM | 0.0537 mL | 0.2687 mL | 0.5373 mL | 1.3433 mL | |
| 80 mM | 0.0403 mL | 0.2015 mL | 0.4030 mL | 1.0074 mL | |
| 100 mM | 0.0322 mL | 0.1612 mL | 0.3224 mL | 0.8060 mL |
Brofaromine Related Classifications
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