DMA trihydrochloride

Name: DMA trihydrochloride
Brand: MedChemExpress
SKU: HY-15621A
Rating: 4.5 (1 reviews)

Cat. No.: HY-15621A Purity: 98.01%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

DMA trihydrochloride is a fluorescent compound (λ_ex=340 nm, λ_em=478 nm).

For research use only. We do not sell to patients.

DMA trihydrochloride Chemical Structure

CAS No. : 2095832-33-8

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in Water ready for reconstitution	USD 92	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 92	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 72	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 108	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 235	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 432	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 792	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of DMA trihydrochloride:

DMA In-stock

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

DMA trihydrochloride is a fluorescent compound (λ_ex=340 nm, λ_em=478 nm).

IC₅₀ & Target

IC50: 3.4 μM (HeLa cell), 5.3 μM (MCF7 cell)^[1]

In Vitro

The newly synthesized bisbenzimidazole derivatives DMA (6c) is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), breast carcinoma cell line (MCF7) and brain glioma cell line (U87) in comparison to Hoechst. In case of MCF7, the IC₅₀ is observed at 5.3 μM for DMA. The IC₅₀ determined in the case of HeLa is 3.4 μM for DMA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

577.93

Formula

C₂₇H₃₁Cl₃N₆O₂

CAS No.

2095832-33-8

Appearance

Solid

Color

Light green to green

SMILES

CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OC)C(OC)=C6)N5)=C4)N3)=C2)CC1.[H]Cl.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 15.9 mg/mL (27.51 mM; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7303 mL	8.6516 mL	17.3031 mL
5 mM	0.3461 mL	1.7303 mL	3.4606 mL
10 mM	0.1730 mL	0.8652 mL	1.7303 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 8.33 mg/mL (14.41 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.01%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (248 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69. [Content Brief]

Cell Assay

Various human tumor cells (U87, HeLa and MCF7) are maintained as monolayer at 37°C in 5% CO₂ using DMEM medium. Approximately 3000-8000 cells/well are seeded in 96-well plates containing 200 μL of medium and incubated for 24 h. The culture medium is replaced by fresh medium containing 1, 10, 50, 100 μM of DMA (6c) and incubated for 24, 48 and 72 h. The cell viability is determined by the MTT assay. The light absorbance is measured using a microplate reader^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.7303 mL	8.6516 mL	17.3031 mL	43.2578 mL
	5 mM	0.3461 mL	1.7303 mL	3.4606 mL	8.6516 mL
	10 mM	0.1730 mL	0.8652 mL	1.7303 mL	4.3258 mL
	15 mM	0.1154 mL	0.5768 mL	1.1535 mL	2.8839 mL
	20 mM	0.0865 mL	0.4326 mL	0.8652 mL	2.1629 mL
	25 mM	0.0692 mL	0.3461 mL	0.6921 mL	1.7303 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.