(S,R,S)-AHPC-PEG6-C4-Cl
Based on 1 Customer Validation
(S,R,S)-AHPC-PEG6-C4-Cl is a conjugate of ligands for E3 and 25-atom-length linker. The connector of linker is Halogen group. (S,R,S)-AHPC-PEG6-C4-Cl incorporates the (S,R,S)-AHPC based VHL ligand and 6-unit PEG linker. (S,R,S)-AHPC-PEG6-C4-Cl is capable of inducing the degradation of GFP-HaloTag7 in cell-based assays.
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- Pureté : 95.97%
- CAS No.: 1835705-59-3
- Formule: C40H63ClN4O10S
- Masse moléculaire:827.47
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Stockage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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HY-103607
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(S,R,S)-AHPC-PEG3-NH2 hydrochlorideE3 Ligase Ligand-Linker Conjugate -
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(S,R,S)-AHPC-PEG2-NH2 hydrochlorideE3 ligase ligand-linker conjugate -
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(S,R,S)-AHPC-PEG4-NH2 hydrochlorideE3 ligase ligand-linker conjugate -
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(S,R,S)-AHPC-PEG4-N3E3 ligase ligand-linker conjugate -
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(S,R,S)-AHPC-(C3-PEG)2-C6-ClE3 ligase ligand-linker conjugate -
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(S,R,S)-AHPC-PEG3-N3E3 ligase ligand-linker conjugate -
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(S,R,S)-AHPC-PEG4-NH2E3 ligase ligand-linker conjugate -
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(S,R,S)-AHPC-PEG2-N3E3 ligase ligand-linker conjugate -
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(S,R,S)-AHPC-PEG3-NH2
Voir tous les produits spécifiques à Isoform E3 Ligase Ligand-Linker Conjugates
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Activité biologique
Description
IC50 & Target
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VHL |
In Vitro
HaloPROTACs are designed to induce ubiquitylation and degradation of HaloTag7 fusion proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1835705-59-3
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Appearance Oil
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Masse moléculaire 827.47
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Formule C40H63ClN4O10S
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Color Light yellow to yellow
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SMILES
O=C([C@H]1N(C([C@H](C(C)(C)C)NC(COCCOCCOCCOCCOCCOCCCCCCCl)=O)=O)C[C@H](O)C1)NCC2=CC=C(C3=C(C)N=CS3)C=C2
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Synonyms
VH032-PEG6-C4-Cl; VHL Ligand-Linker Conjugates 10; E3 ligase Ligand-Linker Conjugates 9
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
Ethanol : 100 mg/mL (120.85 mM; Need ultrasonic)
DMSO : ≥ 100 mg/mL (120.85 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Directly Induced Neuron Culture
Directly induced neuron culture converts somatic cells, most commonly fibroblasts, into induced neurons without passing through a pluripotent or neural progenitor stage; classic evidence shows that mouse fibroblasts can be converted by Ascl1, Brn2/Pou3f2, and Myt1l, human fibroblasts can be converted by defined neuronal transcription factors, and human fibroblasts can also be converted by miR-9/9-124 with neurogenic or subtype-specifying transcription factors. The readout is acquisition of neuronal identity and function, assessed by neuronal morphology, neuronal markers such as Tuj1/βIII-tubulin, MAP2, synapsin, and subtype markers when relevant, together with functional assays such as action-potential firing, synaptic activity, and electrophysiology.
Pureté et documentation
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Fiche technique (269 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 1.2085 mL | 6.0425 mL | 12.0850 mL | 30.2126 mL |
| 5 mM | 0.2417 mL | 1.2085 mL | 2.4170 mL | 6.0425 mL | |
| 10 mM | 0.1209 mL | 0.6043 mL | 1.2085 mL | 3.0213 mL | |
| 15 mM | 0.0806 mL | 0.4028 mL | 0.8057 mL | 2.0142 mL | |
| 20 mM | 0.0604 mL | 0.3021 mL | 0.6043 mL | 1.5106 mL | |
| 25 mM | 0.0483 mL | 0.2417 mL | 0.4834 mL | 1.2085 mL | |
| 30 mM | 0.0403 mL | 0.2014 mL | 0.4028 mL | 1.0071 mL | |
| 40 mM | 0.0302 mL | 0.1511 mL | 0.3021 mL | 0.7553 mL | |
| 50 mM | 0.0242 mL | 0.1209 mL | 0.2417 mL | 0.6043 mL | |
| 60 mM | 0.0201 mL | 0.1007 mL | 0.2014 mL | 0.5035 mL | |
| 80 mM | 0.0151 mL | 0.0755 mL | 0.1511 mL | 0.3777 mL | |
| 100 mM | 0.0121 mL | 0.0604 mL | 0.1209 mL | 0.3021 mL |
(S,R,S)-AHPC-PEG6-C4-Cl Related Classifications
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