Flavin adenine dinucleotide disodium salt
Based on 10 publication(s) in Google Scholar
Flavin adenine dinucleotide (FAD) disodium salt is a redox cofactor, more specifically a prosthetic group of a protein, involved in several important enzymatic reactions in metabolism.
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- Purity : 99.72%
- CAS No.: 84366-81-4
- 화학식: C27H31N9Na2O15P2
- 분자량:829.51
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보관:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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HY-F0001
NADH disodium saltReduced Coenzyme -
HY-B0964
Riboflavin phosphate sodiumEndogenous Metabolite -
HY-F0003
NADPH tetrasodium saltCofactor -
HY-B0606
Diquafosol tetrasodiumP2Y2 Receptor Agonist -
HY-113324
NADPHReducing Agent -
HY-B0456
RiboflavinVitamin B2 -
HY-B1654
Flavin adenine dinucleotideRedox Cofactor -
HY-B2176
ATPEndogenous Metabolite -
HY-B0445
NAD+Endogenous Metabolite -
HY-N7122
ThymopentinActive Peptide -
HY-15917
DL-DithiothreitolReducing Agent -
HY-F0002
NADP sodium saltRedox Coenzyme
Publications Citing Use of MedChemExpress (MCE) Flavin adenine dinucleotide disodium salt
More- Cell Metab. 2026 Mar 3;38(3):565-581.e5. [Abstract]
- Cancer Res. 2026 Jul 15;86(14):3436-3458. [Abstract]
- Nat Commun. 2025 Oct 29;16(1):9547. [Abstract]
- Nat Commun. 2025 Jan 2;16(1):212. [Abstract]
- J Med Chem. 2024 Nov 14;67(21):19874-19888. [Abstract]
- Microb Biotechnol. 2025 Oct;18(10):e70254. [Abstract]
- Int J Mol Sci. 2025 Feb 6;26(3):1381. [Abstract]
- ACS Chem Biol. 2026 Jul 2.
- Res Sq. 2025 Oct 3.
- SSRN. 2025 Sep 11.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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RT-PCR
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Bio/Physico-chemical Assay
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RT-PCR
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
More
Biological Activity
제품 설명
IC50 & Target
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Microbial Metabolite |
Human Endogenous Metabolite |
In Vitro
Poly(Flavin adenine dinucleotide, FAD) characterized by an additional polymer-type redox reaction is a highly effective electrocatalyst for NADH oxidation: operating at the lowest potentials reported for NADH transducers (0.00 V, pH 7.4), poly(FAD) is characterized by the electrochemical rate constant of 1.8 ± 0.6×10-3 cm/s, which is at the level of the NADH mass-transfer constant. Poly(FAD)-modified electrodes are characterized by the dramatically improved stability and, is the most advantageous NADH transducers for analytical chemistry[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 84366-81-4
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Appearance Solid
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분자량 829.51
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화학식 C27H31N9Na2O15P2
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Color Yellow to orange
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SMILES
NC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](COP(OP(OC[C@@H](O)[C@@H](O)[C@@H](O)CN4C(C(C(N5)=O)=NC6=C4C=C(C)C(C)=C6)=NC5=O)(O[Na])=O)(O[Na])=O)O3
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Synonyms
FAD disodium salt; FAD-Na2
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (10)
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Journal Impact Factor
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Most Recent
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Cell Metab
Microbial metabolite FAD mobilizes adipocyte lipid remodeling to enhance cancer immunotherapy efficacy. [Abstract]2026 Mar 3;38(3):565-581.e5. PMID: 41570815 -
Cancer Res
TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma. [Abstract]2026 Jul 15;86(14):3436-3458. PMID: 41974002 -
Nat Commun
FAD synthase confers ferroptosis resistance and restrains CD8+ T cell recruitment in hepatocellular carcinoma. [Abstract]2025 Oct 29;16(1):9547. PMID: 41162372
Flavin adenine dinucleotide disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Oct 29;16(1):9547. [Abstract]
Hep3B and HCCLM3 cells treated with Riboflavin (VB2; 10 µM), Riboflavin phosphate sodium (FMN; 10 µM), Flavin adenine dinucleotide (FAD; 10 µM), or PBS (Ctrl) for 48 h, followed by CCK-8 assay.
Flavin adenine dinucleotide disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Oct 29;16(1):9547. [Abstract]
Relative mRNA levels of interferon-stimulated genes (ISG15, IFIT1, IFNB1) and chemokines (CCL5, CXCL9, CXCL10) in Hep3B cells (n = 3 biological replicates). Flavin adenine dinucleotide (FAD), 20 µM for 48 h; H-151 (STING inhibitor), 1 μM for 12 h.
Flavin adenine dinucleotide disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Oct 29;16(1):9547. [Abstract]
Levels of ROS and lipid peroxidation in Hep3B cells (n = 3 biological replicates). Flavin adenine dinucleotide (FAD), 20 µM for 48 h.
Flavin adenine dinucleotide disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Oct 29;16(1):9547. [Abstract]
GR activity, GSH levels, and GSH/GSSG ratio in Hep3B cells (n = 4 biological replicates). Flavin adenine dinucleotide (FAD), 20 µM for 48 h.
Flavin adenine dinucleotide disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Oct 29;16(1):9547. [Abstract]
Hep3B cells with FADS-knockdown treated with VB2 (10 µM), FMN (10 µM), Flavin adenine dinucleotide (FAD;10 µM), or PBS (Ctrl) for 48 h, followed by CCK-8 assay.
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Nat Commun
Mitochondrial-cytochrome c oxidase II promotes glutaminolysis to sustain tumor cell survival upon glucose deprivation. [Abstract]2025 Jan 2;16(1):212. PMID: 39747079
Flavin adenine dinucleotide disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 2;16(1):212. [Abstract]
H1299-shMT-CO2 cells were treated with 20 μM Flavin adenine dinucleotide disodium salt (FAD) for 48 h, followed by CHIP-qPCR analyses (n = 3 independent experiments).
Flavin adenine dinucleotide disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 2;16(1):212. [Abstract]
H1299 cells were treated with an indicated concentration of Flavin adenine dinucleotide disodium salt (FAD) for 48 h, followed by western blot analyses.
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J Med Chem
Target Ligand Separation and Identification of Isoforsythiaside as a Histone Lysine-Specific Demethylase 1 Covalent Inhibitor Against Breast Cancer Metastasis. [Abstract]2024 Nov 14;67(21):19874-19888. PMID: 39499621 -
Microb Biotechnol
Novel Anthraquinone Chlorination Contributes to Pigmentation and ATP Formation in Thermomyces dupontii. [Abstract]2025 Oct;18(10):e70254. PMID: 41123250 -
Int J Mol Sci
Discovery of TRPV4-Targeting Small Molecules with Anti-Influenza Effects Through Machine Learning and Experimental Validation. [Abstract]2025 Feb 6;26(3):1381. PMID: 39941149 -
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용액&용해도
In Vitro:
H2O : 33.33 mg/mL (40.18 mM; Need ultrasonic)
DMSO : 5 mg/mL (6.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
순도&문서
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
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- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sugiyama S, et al. Protection of chlorpromazine-induced arrhythmia by flavin-adenine-dinucleotide in canine heart. Jpn Heart J. 1979 Sep;20(5):657-65. [Content Brief]
[2]. Karyakin AA, et al. Electropolymerized flavin adenine dinucleotide as an advanced NADH transducer. Anal Chem. 2004 Apr 1;76(7):2004-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.2055 mL | 6.0277 mL | 12.0553 mL | 30.1383 mL |
| 5 mM | 0.2411 mL | 1.2055 mL | 2.4111 mL | 6.0277 mL | |
| H2O | 10 mM | 0.1206 mL | 0.6028 mL | 1.2055 mL | 3.0138 mL |
| 15 mM | 0.0804 mL | 0.4018 mL | 0.8037 mL | 2.0092 mL | |
| 20 mM | 0.0603 mL | 0.3014 mL | 0.6028 mL | 1.5069 mL | |
| 25 mM | 0.0482 mL | 0.2411 mL | 0.4822 mL | 1.2055 mL | |
| 30 mM | 0.0402 mL | 0.2009 mL | 0.4018 mL | 1.0046 mL | |
| 40 mM | 0.0301 mL | 0.1507 mL | 0.3014 mL | 0.7535 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Flavin adenine dinucleotide disodium salt Related Classifications
HY-B1654A Related Classifications
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