2. Anti-infection
  3. HIV Reverse Transcriptase
  4. Tenofovir alafenamide fumarate

Tenofovir alafenamide fumarate  (Synonyms: GS-7340 fumarate)

Cat. No.: HY-15232A Purity: 99.87%
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Tenofovir alafenamide fumarate (GS-7340 fumarate) is an investigational oral proagent of Tenofovir. Tenofovir is a HIV-1 nucleotide reverse transcriptase inhibitor.

For research use only. We do not sell to patients.

CAS No. : 379270-38-9

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10 mM * 1 mL in DMSO
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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Tenofovir alafenamide fumarate:

Tenofovir alafenamide fumarate Related Antibodies

Top Publications Citing Use of Products

    Tenofovir alafenamide fumarate purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2021 Oct 11;13(10):1656.  [Abstract]

    The activity of rhCES1 against TAF and SBV hydrolysis was assessed. 40 ng/µL rhCES1 was incubated with 20 µM TAF or SBV at 37 °C for 5 min and 20 min, respectively.

    Tenofovir alafenamide fumarate purchased from MedChemExpress. Usage Cited in: J Gastroenterol. 2020 Apr;55(4):441-452  [Abstract]

    HepG2.2.15.7 cells were cultured for 6 days with or without a specified concentration of PDM2, and treated with or without Tenofovir alafenamide (TAF). Cell viability was determined using the MTT assay.

    View All HIV Isoform Specific Products:

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Tenofovir alafenamide fumarate (GS-7340 fumarate) is an investigational oral proagent of Tenofovir. Tenofovir is a HIV-1 nucleotide reverse transcriptase inhibitor.

    IC50 & Target

    HIV-1

     

    HIV-2

     

    Cellular Effect
    Cell Line Type Value Description References
    HepG2 CC50
    >50 μM
    Compound: TAF-SEE
    Cytotoxicity against human HepG2-hNTCP cells incubated for 3 days by XTT assay
    Cytotoxicity against human HepG2-hNTCP cells incubated for 3 days by XTT assay
    		[PMID: 34713696]
    MT4 CC50
    35 μM
    Compound: TAF-SEE
    Cytotoxicity against in human MT4 cells after 5 days by XTT assay
    Cytotoxicity against in human MT4 cells after 5 days by XTT assay
    		[PMID: 34713696]
    In Vitro

    Tenofovir alafenamide fumarate (GS-7340 fumarate) antiviral activities are similar across all cell types, ranging from 5 to 7 nM, while the CC50 varies from 4.7 to 42 μM for MT-4 and MT-2 cells, respectively. The antiviral activity of Tenofovir alafenamide fumarate is evaluated against a panel of HIV-1 and HIV-2 isolates, including HIV-1 group M subtypes A to G, as well as group N and O isolates. Overall, for the 29 primary HIV-1 isolates tested in PBMCs, Tenofovir alafenamide fumarate EC50s range from 0.1 to 12 nM, with a mean EC50 of 3.5 nM compared to a mean EC50 of 11.8 nM for Tenofovir alafenamide fumarate, which is used as an internal control. For the HIV-2 isolates, the mean EC50s are 1.8 nM for Tenofovir alafenamide fumarate and 6.4 nM for AZT[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tenofovir alafenamide fumarate Related Antibodies
    In Vivo

    Tenofovir alafenamide fumarate (GS-7340 fumarate) is an amidate prodrug of Tenofovir with good oral bioavailability and increases plasma stability compared to Tenofovir disoproxil fumarate (TDF)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    592.54

    Formula

    C25H33N6O9P
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=NC=NC2=C1N=CN2C[C@@H](C)OC[P@](OC3=CC=CC=C3)(N[C@@H](C)C(OC(C)C)=O)=O.O=C(O)/C=C/C(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 36 mg/mL (60.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 25 mg/mL (42.19 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6876 mL 8.4382 mL 16.8765 mL
    5 mM 0.3375 mL 1.6876 mL 3.3753 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 6.67 mg/mL (11.26 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.87%

    SDS (643 KB)

    COA (258 KB) HNMR (279 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.6876 mL 8.4382 mL 16.8765 mL 42.1912 mL
    5 mM 0.3375 mL 1.6876 mL 3.3753 mL 8.4382 mL
    10 mM 0.1688 mL 0.8438 mL 1.6876 mL 4.2191 mL
    15 mM 0.1125 mL 0.5625 mL 1.1251 mL 2.8127 mL
    20 mM 0.0844 mL 0.4219 mL 0.8438 mL 2.1096 mL
    25 mM 0.0675 mL 0.3375 mL 0.6751 mL 1.6876 mL
    30 mM 0.0563 mL 0.2813 mL 0.5625 mL 1.4064 mL
    40 mM 0.0422 mL 0.2110 mL 0.4219 mL 1.0548 mL
    DMSO 50 mM 0.0338 mL 0.1688 mL 0.3375 mL 0.8438 mL
    60 mM 0.0281 mL 0.1406 mL 0.2813 mL 0.7032 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Tenofovir alafenamide fumarate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tenofovir alafenamide379270-38-9GS-7340GS7340GS 7340HIVReverse TranscriptaseHuman immunodeficiency virusInhibitorinhibitorinhibit

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