Hexa-His
Based on 1 Customer Validation
Hexa-His (6X His Tag) is a commonly used affinity tag made up of six histidine residues. HEXA-HIS can bind to affinity chromatography media containing transition metal ions like nickel (Ni2+) or cobalt (Co2+), making it useful for protein purification.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 64134-30-1
- Formula: C36H44N18O7
- Molecular Weight:840.85
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Hexa-His for Protein Purification and Protein Analysis[2]
(1) Construct a plasmid containing the target gene and Hexa-His tag, and introduce it into E. coli.
(2) Select positive clones and grow them in LB medium until the OD600 is about 0.6-0.8, then add IPTG (HY-15921, isopropyl β-D-thiogalactoside) as an inducer and continue to incubate for a few hours to induce the expression of the target protein.
(3) Collect the bacterial cells, resuspend them in lysis buffer, and sonicate to lyse the cells, then centrifuge to remove debris and obtain the supernatant.
(4) Prepare the Ni-NTA affinity chromatography column and slowly pass the lysate supernatant through the Ni-NTA column to bind the CTB protein with the Hexa-His tag to the column.
(5) After protein adsorption, wash the resin with 10 column volumes of 60 mM imidazole binding buffer. Elute the target gene with the same solution containing 1 M imidazole using 5 column volumes. Collect the protein and analyze it by 15% SDS-PAGE.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 64134-30-1
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Appearance Solid
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Molecular Weight 840.85
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Formula C36H44N18O7
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Color White to off-white
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Synonyms
6X His Tag
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Sequence
His-His-His-His-His-His
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Sequence Shortening
HHHHHH
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 50 mg/mL (59.46 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (118.93 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Watly J, et al. Insight into the coordination and the binding sites of Cu(2+) by the histidyl-6-tag using experimental and computational tools. Inorg Chem. 2014 Jul 7;53(13):6675-83. [Content Brief]
[2]. Ana Paula de Mattos Arêas, et al. Synthesis of cholera toxin B subunit gene: cloning and expression of a functional 6XHis-tagged protein in Escherichia coli.Protein Expr Purif.2002 Aug;25(3):481-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.1893 mL | 5.9464 mL | 11.8927 mL | 29.7318 mL |
| 5 mM | 0.2379 mL | 1.1893 mL | 2.3785 mL | 5.9464 mL | |
| 10 mM | 0.1189 mL | 0.5946 mL | 1.1893 mL | 2.9732 mL | |
| 15 mM | 0.0793 mL | 0.3964 mL | 0.7928 mL | 1.9821 mL | |
| 20 mM | 0.0595 mL | 0.2973 mL | 0.5946 mL | 1.4866 mL | |
| 25 mM | 0.0476 mL | 0.2379 mL | 0.4757 mL | 1.1893 mL | |
| 30 mM | 0.0396 mL | 0.1982 mL | 0.3964 mL | 0.9911 mL | |
| 40 mM | 0.0297 mL | 0.1487 mL | 0.2973 mL | 0.7433 mL | |
| 50 mM | 0.0238 mL | 0.1189 mL | 0.2379 mL | 0.5946 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Hexa-His Related Classifications
HY-P0294 Related Classifications
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